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Apache Tomcat

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    Effects of different cultivation measures on the structure and function of fungal and bacterial communities in the rhizosphere soil of cigar tobacco
    SHEN Jun-ru, HE Guo-you, TANG Xu-bing, REN Long-hui, FANG Bao, ZHEN An-zhong, KONG Chui-si
    HUBEI AGRICULTURAL SCIENCES    2025, 64 (2): 6-12.   DOI: 10.14088/j.cnki.issn0439-8114.2025.02.002
    Abstract120)      PDF (2758KB)(44)       Save
    Using high-throughput sequencing technology, the changes of fungal and bacterial communities at the phylum and genus level in the rhizosphere soil under different cultivation methods of cigar tobacco were studied, the effects of cultivation methods on the structural diversity of fungal and bacterial communities were discussed, and functional gene pre-analysis was carried out. The results showed that the richness and diversity of fungal community in rhizosphere soil were significantly decreased under different cultivation patterns compared with the conventional cultivation (CK), and the difference of the former was statistically significant(P<0.05). Cultivation measures did not change the main composition of fungi and bacteria at phylum level, but could significantly recruit beneficial bacteria in the rhizosphere soil. The dominant fungal phyla in the rhizosphere soil were Ascomycota and Basidiomycota, and the dominant fungal genera were Fusarium, Alternaria and Penicillium. The dominant bacterial phyla were Proteobacteria and Actinobacteriota (except CK), but the dominant bacterial genera varied under different treatments. The effect of Trichoderma asperellum or bio-carbon organic fertilizer on rhizosphere fungi and bacteria communities was more obvious.
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    Effect of biochar particle size on photocatalytic degradation of endogenous polycyclic aromatic hydrocarbons
    GE Yun-yu, SHEN Xiu-li, MENG Hai-bo, FENG Jing, CONG Hong-bin, WEN Feng-rui, ZHANG Wen-jing
    HUBEI AGRICULTURAL SCIENCES    2025, 64 (2): 13-20.   DOI: 10.14088/j.cnki.issn0439-8114.2025.02.003
    Abstract103)      PDF (5255KB)(15)       Save
    To explore and optimize the technology and process of photolytic removal of endogenous polycyclic aromatic hydrocarbons(PAHs) in biochar, this study focused on corn stover biochar. Biochar with different particle sizes (>1.0 mm, 0.5~1.0 mm, 0.001~0.5 mm) was prepared, and TiO2-photocatalytic degradation experiments were conducted to systematically analyze the characteristics of the biochar and the efficacy of degrading and removing endogenous PAHs with different particle sizes. The results showed that the endogenous PAHs content in corn stover biochar was 76.1 mg/kg, predominantly comprising 4-ring and 5-ring PAHs, significantly exceeding the limit value for basic biochar PAHs (20 mg/kg) set by the International Biochar Initiative (IBI). TiO2-photocatalysis could significantly reduce the content of endogenous PAHs in biochar. After photocatalytic degradation, the endogenous PAHs content in the three particle sizes of biochar ranged from 3.3 to 14.9 mg/kg, with degradation rates for total PAHs ranging from 55.2% to 95.6%. The maximum degradation rate for high-ring PAHs reached 97.1%, while low-ring PAHs reached 62.0%. The 0.001~0.5 mm particle size biochar exhibited the highest degradation rate of 95.6% after 40 minutes of light exposure, and the toxicity equivalent value decreased from 9.096 mg/kg to 0.088 mg/kg, significantly below the thresholds set by the European Biochar Certification(4 mg/kg) and the International Biochar Initiative(6 mg/kg). The study demonstrated that TiO2-photocatalysis effectively degraded endogenous PAHs in biochar of different particle sizes, ensuring the safety of biochar and its products for application in agricultural fields.
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