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    Effects of molasses addition and pretreatment on forage quality of rape straw micro-storage
    WANG Yan, LIU Heng-heng, WU Yu-peng, LIU Bin, ZHANG Yan-yan, XU Zhi-yu
    HUBEI AGRICULTURAL SCIENCES    2025, 64 (2): 138-143.   DOI: 10.14088/j.cnki.issn0439-8114.2025.02.021
    Abstract78)      PDF (2539KB)(10)       Save
    Effects of molasses addition (0%, 5% and 10%) and pretreatment (high temperature cooking, aerobic pretreatment and high temperature cooking combined with aerobic pretreatment) on forage quality of rape straw micro-storage were analyzed. The results showed that within the high-temperature cooking pretreatment, the CP and WSC content of ensiled rape straw feed increased from 3.55% to 7.07% and 0.56% to 1.23% respectively, with the increase of molasses addition. DM content decreased from 33.02%, and pH declined from 4.44 to 4.08. While ADF and NDF content decreased by 14.16% and 12.16% respectively, with the increase of molasses addition. Within the aerobic pretreatment, the CP and DM contents of ensiled rape straw feed increased slightly with the increase of molasses addition, while pH declinced from 5.38 to 4.02, and WSC, ADF and NDF content decreased by 0.46%, 7.66% and 7.43%, respectively. Within the combination of high temperature cooking and aerobic pretreatment, the CP and WSC contents of ensiled rape straw feed increased from 6.66% to 11.06% and 0.49% to 0.97% respectively, with the increasing of molasses addition. While non-significant change was detected for DM, ADF and NDF contents. Therefore, the addition of molasses could effectively improve the fermentation quality of rape straw micro-storage, no matter what kinds of pretreatment adopted. In addition, the optimal amount of molasses addition was 10% based on the fresh amount of rape straw.
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    Protection of Gynostemma pentaphyllum polysaccharide on oxidative damage of ovary in mice under heat stress
    CHEN Lei-xiao, HE Huan, OUYANG Lu, MOU Rong-feng, LAI Peng-yu, WANG Xiao-li
    HUBEI AGRICULTURAL SCIENCES    2025, 64 (2): 144-149.   DOI: 10.14088/j.cnki.issn0439-8114.2025.02.022
    Abstract69)      PDF (4760KB)(8)       Save
    In order to explore the protective effect of different doses of Gynostemmapentaphyllum polysaccharides (GPP) on oxidative damage of ovary in mice caused by heat stress, enzyme-linked immunosorbent assay (ELISA), immunohistochemical techniques and other research methods were used to observe and analyze the body weight and ovarian index, the number of follicles at all levels, the maximum diameter and the thickness of ovarian cortex, various reproductive hormones in ovary, the level of estrogen receptor β expression, antioxidant capacity in blood serum and the apoptosis rate of ovarian granulosa cells. The results showed that compared with group HS, the weight of mice in different doses of GPP was significantly higher (P<0.05);the number of primordial follicles in group GPP-H was significantly higher (P<0.05); the number of atretic follicles in groups GPP-L and GPP-H was significantly lower (P<0.05);the thickness of ovarian cortex, the maximum diameters of primordial follicle, primary follicle and corpus luteum in different doses of GPP were significantly larger (P<0.05); the secretion of GnRH, E2 and P4 in different doses of GPP was significantly higher, the secretion of FSH in group GPP-H was significantly higher (P<0.05); GSH-Px activity indifferent doses of GPP was significantly higher (P<0.05), T-SOD activity in group GPP-M was significantly higher (P<0.05), the content of MDA in different doses of GPP was significantly lower (P<0.05). In conclusion, GPP could improve the endocrine function of pituitary and ovary, restore and protect the ovarian reserve capacity, enhance the activity of enzyme antioxidant system, and alleviate the oxidative damage of ovary caused by heat stress by exerting the steroid-like effect of ER agonist, in which GPP-H worked most efficiently.
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