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Apache Tomcat

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    Isolation, identification and biological characterization of a strain of Riemerella anatipestifer
    WANG Bo-tao, GUO Yun-qing, ZHANG Teng-fei, ZHANG Wen-ting, LU Qin, HU Qiao, LUO Qing-ping, ZHAI Xin-guo
    HUBEI AGRICULTURAL SCIENCES    2024, 63 (9): 156-160.   DOI: 10.14088/j.cnki.issn0439-8114.2024.09.026
    Abstract145)      PDF (6081KB)(11)       Save
    In order to determine the cause of acute death of ducks in a large-scale duck farm in Xiangyang City, Hubei Province in 2022, the pathogenic bacteria were isolated, and the biochemical characteristics, drug resistance, animal regression tests and whole genome sequencing of the isolated strain were identified. The results showed that only Riemerella anatipestifer was isolated and identified as positive in acute dead duck pathogens. The drug susceptibility results showed that the isolated strains had high sensitivity to antibiotics and were resistant to ciprofloxacin and ofloxacin. The results of animal regression experiments showed that isolated strains could cause acute death of ducklings, typical symptoms of serositis were seen on autopsy. Histopathological sections showed obvious fibrous exudation of the heart and liver envelope, inflammatory infiltration of cardiomyocytes, a significant decrease in the number of lymphocytes in the spleen parenchyma, and more vascular congestion and expansion in brain tissue. Virulence assays showed that the half lethal dose of isolated strains was 3.06×106 CFU/mL.
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    Habitat differences in seed dispersal distances by forest rodents in Zhang Guangcai mountains
    LI Dian-wei, ZHANG Cheng-zhi, GAO Ming, CAO Yu-wei, CHANG Shi-qi, XU Meng-hao, WANG Yang
    HUBEI AGRICULTURAL SCIENCES    2024, 63 (9): 161-166.   DOI: 10.14088/j.cnki.issn0439-8114.2024.09.027
    Abstract141)      PDF (3617KB)(32)       Save
    In order to explore the relationship between habitat and rodent dispersal to seeds,using the plastic label marking method,Pinus koraiensisP.k), Corylus mandshuricaC.m), and Quercus mongolicaQ.m) seeds were used as materials for the experiments, from September to November 2020, and four habitats (broad-leaved forest, forest edge, mixed forest, artificial pine forest) in Zhangguangcai Mountains, Heilongjiang Province were selected as experimental sites. The results showed that, ①The dispersal distance of plant seeds in the artificial pine forest(2.92±2.24 m)provided a better hiding environment for rodents, with the least anthropogenic disturbance,which was much better than that in broad-leaved forest(3.69±1.92 m), forest edge(3.46±2.29 m) and mixed forest(3.43±2.25 m)(P<0.01). The differences in seed dispersal distances among the remaining three forest types were not significant. ②The dispersal distances of the three seeds were (4.38±3.62) m for P.k, (3.29±2.70) m for C.m, and (2.16±2.66) m for Q.m, under conditions without habitat differences. The dispersal distance of P.k, C.m and Q.m seeds was concentrated within the range of 1~6 m. It indicated that the distance of seed dispersal by rodents was related to the concealability of the habitat and the degree of artificial interference, the better the state of concealment in the habitat, the smaller the dispersal distance, the more the artificial interference, the greater the dispersal distance. Rodents differed in their diffuse selection of plant seeds. The difference in dispersal distances between P.k and C.m was not significant when habitats were not distinguished, but both were significantly greater than Q.m.
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