|
|
Establishment of quality standard for freeze-dried tablets of Polygonatum sibiricum Delar. ex Redoute and study on the anti-tumor activity of diosgenin
XU Yu-sheng, GAO Ming-ju, ZHAO Ning-dong, HUANG Zai-qiang, TAIN Ying-qiu, HU Zhan-yu, YU Zheng-yong
HUBEI AGRICULTURAL SCIENCES
2022, 61 (13):
133-137.
DOI: 10.14088/j.cnki.issn0439-8114.2022.13.025
The freeze-dried powder samples of Polygonatum sibiricum Delar. ex Redoute from 4 different producing areas were used as materials, with reference to the quality standard of Polygonatum sibiricum Delar. ex Redoute in the 2020 edition of Chinese Pharmacopoeia. The total ash content was determined by the total ash determination method, the moisture content was determined by the drying method, the extract content was determined by the hot immersion method, the total sugar content was determined by the 0.2% anthrone-sulfuric acid method, and the diosgenin was determined by the HPLC method. The antitumor activities of diosgenin against A431 （human epidermal cancer cells）, H1975 （human lung adenocarcinoma cells） and Ramos （human B lymphocyte tumor cells） were investigated by the MTT method. The results showed that the moisture content of the samples was 2.8%~4.7% （not more than 18.0%）, the total ash content was 1.9%~3.4%（not more than 4.0%）, the alcohol soluble extract was 72.99%~78.99%, （not less than 45.0%）, and the total sugar was 7.95%~9.94% （not less than 7.0%）. The lowest content of diosgenin was 0.18%, and diosgenin was significantly resistant to A431. The established content determination method was simple, accurate and reproducible.
Reference |
Related Articles |
Metrics
|
|
