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Published in last 1 year |  In last 2 years |  In last 3 years |  All Please wait a minute... For Selected: Download Citations EndNote Reference Manager ProCite BibTeX RefWorks Toggle Thumbnails Select Study on RNA difference of Xinjiang red meat apple extracted by different methods TANG Lei, TONG Pan-pan, ZHANG Ya-ruo, WANG Fang, TANG Zhang-hu, LI Wen-hui, YUAN Zu-sheng, WANG Jiang-bo HUBEI AGRICULTURAL SCIENCES    2020, 59 (3): 144-146.   DOI: 10.14088/j.cnki.issn0439-8114.2020.03.032 Abstract （429）      PDF （1586KB）（170）       Knowledge map    Save The total RNA extraction and purification kit， Trnzol method and improved CTAB method were used to extract the total RNA， from the pulp， pericarp， petals and leaves of red meat apple Kizi Alma， and the results of yield， quality and integrity of the three methods were compared. The final results showed that the purity of RNA extracted by Trnzol method was low， the degradation phenomenon， and the pollution of DNA， protein， the yield of pulp and pericarp was not ideal， but the RNA extracted by improved CTAB method and kit method was rarely degraded. The 28 s rRNA and 18 s rRNA bands were clear， the OD260/280 of petals and leaves was between 1.8 and 2.5， the yield of RNA extracted by raw reagent kit method was high， the ratio of OD260/280 and OD260/230 was between 2.0 and 2.5， and the integrity of the bands was good. Compared with the three methods， the clarity， yield and quality of RNA bands extracted by modified CTAB method and TrNzol method were lower than those by raw reagent kit method. Reference | Related Articles | Metrics Select Cloning and bioinformatics analysis on nifH gene of endophytic nitrogen-fixing bacteria Klebsiella variicola GN02 isolated from Pennisetum giganteum LIN Biao-sheng, ZHANG Huan-xing, HE Yu-qin, LUO Mao-chun, LIN Zhan-xi HUBEI AGRICULTURAL SCIENCES    2020, 59 (3): 147-153.   DOI: 10.14088/j.cnki.issn0439-8114.2020.03.033 Abstract （449）      PDF （2460KB）（165）       Knowledge map    Save To research the bioinformatics on the nifH gene of endophytic nitrogen-fixing bacteria Klebsiella variicola GN02 isolated from Pennisetum giganteum, the nifH gene of GN02 strain was cloned and its integrity was verified, and bioinformatics prediction and analysis of nifH gene were carried out by a variety of online biological software. The results showed that the nifH gene of GN02 strain had a complete ORF, length of 882 bp and encoded 293 amino acid, and the gene accession numbers registered in NCBI GenBank was MK131264. Bioinformatics analysis showed that the nifH gene of GN02 strain was highly conserved and had more than 95% homology with the nucleotide sequence and amino acid sequence of nifH gene from other species. The total relative molecular weight of nifH protein was 31 970.73 and the theoretical isoelectric point pI was 4.72. The transmembrane model inclined to the inward to outward transmembrane of N-end, and the whole polypeptide chain was hydrophilic. nifH protein had two glycosylation sites but no signal peptide secretion, which may had a helical crimp structure. The prediction of secondary structure showed that α-helix and irregular curl were the main structural elements in the polypeptide chain of nifH protein, and the extended chain was scattered in the whole protein. Three-dimensional homologous modeling of nifH protein can be carried out in the database. Reference | Related Articles | Metrics