响应面法优化链霉菌LG-9发酵条件及对棉花黄萎病菌的抑菌作用

doi:10.14088/j.cnki.issn0439-8114.2018.08.019

湖北农业科学 ›› 2018, Vol. 57 ›› Issue (8): 71-76.doi: 10.14088/j.cnki.issn0439-8114.2018.08.019

响应面法优化链霉菌LG-9发酵条件及对棉花黄萎病菌的抑菌作用

陈明¹, 穆凯热姆·阿卜来提¹, 刘政², 王晓东¹

1.石河子大学农学院/绿洲农作物病害防控重点实验室,新疆石河子 832000;
2.石河子农垦科学院植物保护研究所,新疆石河子 832000

收稿日期:2017-11-29 发布日期:2018-04-25
通讯作者: 王晓东（1975-）,新疆石河子人,副教授,博士,主要从事植物病害生物防治研究,（电话）13070092765（电子信箱）wxd_agr@shzu.edu.cn。
作者简介:陈明（1991-）,男,甘肃张掖人,在读硕士研究生,研究方向为植物病害生物防治,（电话）13279931200（电子信箱）1074574330@qq.com
基金资助:
国家自然科学基金项目（31360452）

Optimization of Fermentation Conditions for Anti-streptomyces LG-9 Using Response Surface Methodology and Its Inhibiton on Verticillium dahliae

CHEN Ming¹, MUKARAM Ablat¹, LIU Zheng², WANG Xiao-dong¹

1.College of Agronomy,Shihezi University/Key Laboratory of Oasis Crop Disease Prevention and Control,Shihezi 832000,Xinjiang,China;
2.Institute of Plant Protection,Shihezi Academy of Agricultural Sciences,Shihezi 832000,Xinjiang,China

Received:2017-11-29 Published:2018-04-25

摘要/Abstract

摘要： 为提高链霉菌菌株LG-9发酵液的抑菌活性,在单因素试验的基础上,利用Minitab软件中的Plackett-Burman设计和响应面分析,以发酵液对棉花黄萎病菌（Verticillium dahliae）的抑菌圈大小为响应值,对链霉菌LG-9的液体发酵条件进行优化。结果表明,菌株LG-9的最佳发酵条件为250 mL三角瓶装样量101.19 mL、培养基初始pH 7.2、接种量7.5%、温度31.32 ℃、转速160 r/min和摇培时间69.02 h。在此条件下的抑菌圈直径大小为22.45 mm,较未优化前提高10.43%。

关键词: 棉花黄萎病菌（Verticillium dahliae）, 链霉菌, 响应面分析, 发酵条件优化

Abstract: In order to improve the antibacterial activity of Streptomyces isolate LG-9,on the basis of single factor test,using the Plackett-Burman experiment design and response surface analysis of Minitab software,with the inhibition circle size of the fermentation broth on Verticillium dahliae as the response value,and the liquid fermentation condition of Streptomyces LG-9 were optimized. The results showed that the optimal fermentation conditions of the strains LG-9 were loading volume of liquid medium 101.19 mL in 250 mL triangle bottle,pH 7.2,inoculation amount of 7.5%,temperature 31.32 ℃,rotate 160 r/min,time 69.02 h. The diameter of the inhibition zone under these conditions was 22.45 mm,which was 10.43% higher than that before optimization.

Key words: Verticillium dahliae, streptomyces, response surface analysis, optimization of fermentation conditions

中图分类号:

陈明, 穆凯热姆·阿卜来提, 刘政, 王晓东. 响应面法优化链霉菌LG-9发酵条件及对棉花黄萎病菌的抑菌作用[J]. 湖北农业科学, 2018, 57(8): 71-76.

CHEN Ming, MUKARAM Ablat, LIU Zheng, WANG Xiao-dong. Optimization of Fermentation Conditions for Anti-streptomyces LG-9 Using Response Surface Methodology and Its Inhibiton on Verticillium dahliae[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(8): 71-76.

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响应面法优化链霉菌LG-9发酵条件及对棉花黄萎病菌的抑菌作用

Optimization of Fermentation Conditions for Anti-streptomyces LG-9 Using Response Surface Methodology and Its Inhibiton on Verticillium dahliae

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