湖北农业科学 ›› 2019, Vol. 58 ›› Issue (9): 114-116.doi: 10.14088/j.cnki.issn0439-8114.2019.09.027

• 检测分析 • 上一篇    下一篇

HPLC测定四川梓潼产葛根和粉葛中四种异黄酮含量的分析

徐炜锋1, 杜春燕1, 徐书宇1, 李琪源1, 苏丽丽1, 杨刚2, 蒋合众1   

  1. 1.西南交通大学生命科学与工程学院,成都 610031;
    2.梓潼富库葛根专业合作社/梓潼天富生物科技有限公司,四川 梓潼 622150
  • 收稿日期:2018-10-23 出版日期:2019-05-10 发布日期:2019-12-05
  • 作者简介:徐炜锋(1996-),男,浙江绍兴人,在读本科生,制药工程专业,(电话)1713550530(电子信箱)524417896@q,com;通信作者蒋合众(1980-),男,重庆铜梁人,副教授,博士,主要从事天然药物化学研究,(电子信箱)jiangzi0Osina.com
  • 基金资助:
    国家重大科技专项(20137X09103002-014); 2017年中医药公共卫生服务补助专项[财社(2017)66号]; 四川省中医药管理局中医药科学技术研究专项(2016Q037); 大学生科研训练计划项目(2017093; 201710613067); 第12期大学生个性化实验项目(GX201812098)

Quantitative determination of 4 isoflavones of Pueraria lobata and Pueraria thomsonii from Zitong in Sichuan province by HPLC

XU Wei-feng1, DU Chun-yan1, XU Shu-yu1, LI Qi-yuan1, SU Li-li1, YANG Gang2, JIANG He-zhong1   

  1. 1.School of Life Science and Engineering,Southwest Jiaotong University,Chengdu 610031,China;
    2.Zitong Professional Cooperative of Fuku Gegen/Zitong Tianfu Biotechnology Co.,Ltd.,Zitong 622150,Sichuan,China
  • Received:2018-10-23 Online:2019-05-10 Published:2019-12-05

摘要: 采用YMC-Pack ODS-A C18色谱柱(250 mm×4.6 mm,5 μm)测定四川梓潼产葛根和粉葛中4种异黄酮(葛根素、3′-甲氧基葛根素、大豆苷和大豆苷元)含量。结果测得葛根素、3′-甲氧基葛根素、大豆苷和大豆苷元的线性范围分别为0.050~0.750、0.010~0.150、0.010~0.150和0.012~0.180 μg,线性关系良好(r>0.999),平均加样回收率为96.52%~100.22%,RSD为0.75%~1.84%。该方法简便、准确、重现性好,适用于葛根中异黄酮含量的测定。梓潼产葛根中葛根素、3′-甲氧基葛根素、大豆苷和大豆苷元的含量明显高于粉葛,且梓潼产葛根和粉葛中大豆苷元含量明显高于其他产地的葛根和粉葛。

关键词: 葛根, 粉葛, 高效液相, 异黄酮, 含量

Abstract: To determine the contents of four isoflavones of Pueraria lobata and Pueraria thomsonii in Sichuan Zitong by HPLC. The chromatographic conditions were YMC-Pack ODS-A C18 column(250 mm×4.6 mm,5 μm). The linear range of puerarin, 3′-methoxy puerarin, daidzin and daidzein were 0.050~0.750, 0.010~0.150, 0.010~0.150 and 0.012~0.180 μg, respectively, and it showed a good linear relationship(r>0.999), the average recovery rate was between 96.52%~100.22%, and RSD was between 0.75%~1.84%(n=6). This method is simple, accurate and reproducible. It is suitable for the determination of isoflavones in Puerarin. The contents of 4 isoflavones of P. lobata are obviously higher than P. thomsonii from Zitong, Sichuan Province. And the content of daidzein in P. lobata and P. thomsonii from Zitong is obviously higher than other habitat origins reported.

Key words: Pueraria lobata, Pueraria thomsonii, HPLC, isoflavone, content

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