不同繁育方式对果桑光合特性和糖代谢的影响

doi:10.14088/j.cnki.issn0439-8114.2019.16.020

湖北农业科学 ›› 2019, Vol. 58 ›› Issue (16): 89-95.doi: 10.14088/j.cnki.issn0439-8114.2019.16.020

不同繁育方式对果桑光合特性和糖代谢的影响

周建华^a, 李勇^b, 于翠^b, 莫荣利^b, 朱志贤^b, 董朝霞^b, 胡兴明^b, 邓文^b

湖北省农业科学院,a.产业处;b.经济作物研究所,武汉 430064

收稿日期:2019-04-15 出版日期:2019-08-25 发布日期:2019-11-12
通讯作者: 邓文（1968-）,男,副研究员,主要从事桑树育种与栽培研究工作,（电子信箱）Dengwen-1@126.com。
作者简介:周建华（1972-）,男,湖北麻城人,助理研究员,主要从事蚕桑生产技术的推广工作,（电话）027-87389769（电子信箱）1062528434@qq.com
基金资助:
国家现代农业产业技术体系建设专项（CARS-18-ZJ0208）

Effects of photosynthetic characteristics and sugar metabolism of fruit mulberry in different breeding methods

ZHOU Jian-hua^a, LI Yong^b, YU Cui^b, MO Rong-li^b, ZHU Zhi-xian^b, DONG Zhao-xia^b, HU Xing-ming^b, DENG Wen^b

a.Department of Industry; b.Cash Crops Research Institute,Hubei Academy of Agricultural Sciences,Wuhan 430064,China

Received:2019-04-15 Online:2019-08-25 Published:2019-11-12

摘要/Abstract

摘要： 为研究不同繁育方式对果桑光合特性和糖积累及相关酶活代谢特征的影响,选择扦插（QC）和嫁接（JJ）2种繁育方式盆栽果桑大10,测定叶片气体交换参数的日变化、动态变化及桑椹不同发育时期的可溶性糖含量、蔗糖代谢相关酶活性变化。结果显示,2种繁育方式果桑净光合速率（Pn）日变化曲线均呈双峰曲线,有明显光合“午休”现象,但QC果桑第二个峰值高于JJ果桑,蒸腾速率（Tr）日变化曲线中均为双峰曲线,Tr最大峰值QC>JJ;表观光能利用效率（LUE）和瞬时水分利用效率（WUE）均为QC>JJ,表观CO₂利用效率（CUE）为JJ>QC。2种繁育方式桑椹不同发育时期糖分含量存在差异,可溶性糖（Tss）含量从红果期呈上升趋势,糖分积累以果糖（Fru）和葡萄糖（Glc）为主,桑椹为蔗糖（Can）和己糖共同积累型果实。SS和SPS活性随发育过程的变化类似。不同繁育方式桑椹NI活性在果实发育后期较前期活跃,光合生理参数与桑椹糖积累及蔗糖代谢相关酶活相关性分析表明,Tss、Fru、Glc均与Pn、LUE、CUE、WUE呈正相关,QC和JJ桑椹糖含量差异表明,不同繁育方式对桑椹光合同化物运输与分配有影响。

关键词: 繁育, 果桑, 光合特性, 糖代谢

Abstract: In order to study the effects of different breeding methods on photosynthetic characteristics, sugar accumulation and related enzyme activities, the cuttings （QC） and grafting （JJ） were selected to breeding fruit mulberry DA10, and the gas exchange parameters of mulberry leaves were determined. Diurnal changes, dynamic changes, and soluble sugar content and sucrose metabolism-related enzyme activities in different developmental stages of mulberry. The results showed that the daily variation curves of net photosynthetic rate（Pn） in the two breeding methods showed a double-peak curve with obvious photosynthetic “noon break” phenomenon, but the second peak of QC fruit mulberry was higher than the JJ fruit mulberry peak, and the transpiration rate（Tr） daily variation curve for the bimodal curve, the maximum peak value of Tr was QC>JJ. The utilization efficiency（LUE） and the instantaneous water use efficiency（WUE） were QC>JJ, and the apparent CO₂ utilization efficiency （CUE） was JJ>QC. There were differences in the content of sugar components in different developmental stages of mulberry. The content of soluble sugar（Tss） increased linearly from red fruit stage. The accumulation of sugar was mainly fructose（Fru） and glucose（Glc）, which was the fruit of sucrose（Can） and hexose accumulation. SS and SPS activities were similar as changes in developmental processes. The activity of mulberry NI in different breeding methods was more active in the whole development stage than in the early stage. The correlation between photosynthetic physiological parameters and mulberry sugar accumulation and sucrose metabolism related enzyme activities showed that Tss, Fruand,Glc were were in positive correlation with Pn,LUE,CUE and WUE. The differences in the content of QC and JJ sugar indicated that different breeding methods had an impact on the transportation and distribution of mulberry light contract compound.

Key words: breeding, fruit mulberry, photosynthetic characteristics, sugar metabolism

中图分类号:

S663.9

周建华, 李勇, 于翠, 莫荣利, 朱志贤, 董朝霞, 胡兴明, 邓文. 不同繁育方式对果桑光合特性和糖代谢的影响[J]. 湖北农业科学, 2019, 58(16): 89-95.

ZHOU Jian-hua, LI Yong, YU Cui, MO Rong-li, ZHU Zhi-xian, DONG Zhao-xia, HU Xing-ming, DENG Wen. Effects of photosynthetic characteristics and sugar metabolism of fruit mulberry in different breeding methods[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(16): 89-95.

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不同繁育方式对果桑光合特性和糖代谢的影响

Effects of photosynthetic characteristics and sugar metabolism of fruit mulberry in different breeding methods

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