水稻糖代谢相关酶和糖类转运蛋白编码基因的鉴定和表达分析

doi:10.14088/j.cnki.issn0439-8114.2019.22.043

湖北农业科学 ›› 2019, Vol. 58 ›› Issue (22): 185-193.doi: 10.14088/j.cnki.issn0439-8114.2019.22.043

水稻糖代谢相关酶和糖类转运蛋白编码基因的鉴定和表达分析

王义杰, 张绍杰, 赖艳, 胡永峰

荆楚理工学院/植物种质资源开发与利用研究所,湖北荆门 448000

收稿日期:2018-08-29 出版日期:2019-11-25 发布日期:2019-12-09
通讯作者: 胡永峰（1983-）,男,湖北荆门人,副教授,博士,主要从事植物分子遗传学研究, （电话）13677246318（电子信箱）feng2602@sina.com。
作者简介:王义杰（1998-）,男,湖北应城人,本科,专业方向为植物科学与技术;共同第一作者,张绍杰（1996-）,男,湖北黄梅人,本科,专业方向为植物科学与技术。
基金资助:
国家自然科学基金项目（31301300）; 湖北省高等学校优秀中青年科技创新团队计划项目（T201621）

Identification and expression analysis of genes encoding carbohydrate metabolism enzymes and sugar transporters

WANG Yi-jie, ZHANG Shao-jie, LAI Yan, HU Yong-feng

Jingchu University of Technology/Institute of Plant Germplasm Resources Exploitation and Utilization,Jingmen 448000,Hubei,China

Received:2018-08-29 Online:2019-11-25 Published:2019-12-09

摘要/Abstract

摘要： 水稻（Oryza sativa L.）子粒淀粉的积累是产量形成的基础,灌浆期叶片通过光合作用合成蔗糖,并运输到子粒为淀粉合成提供原料,该过程中所涉及的代谢相关酶和运输相关蛋白已有研究报道。对卡尔文循环、蔗糖合成与降解、淀粉合成与降解等糖代谢相关的酶以及糖类转运蛋白编码基因进行了系统分析,共发现糖代谢相关蛋白编码基因148个和糖类转运蛋白编码基因102个,其中有228个基因已有文献报道,新鉴定基因22个。表达谱分析发现其中的部分基因表达具有组织特异性,与其功能有密切的关系。同时还发现许多基因受逆境胁迫影响表达发生变化,表明这些基因可能在水稻抗逆境胁迫中具有重要的作用。

关键词: 水稻（Oryza sativa L.）, 糖代谢, 糖类转运蛋白, 产量形成

Abstract: Starch accumulation in rice（Oryza sativa L.） seeds is the basis of rice yield formation. In filling stage sucrose is synthesized in rice leaves by photosynthesis, and then transported to seeds providing materials for starch synthesis. Several enzymes and transporters in this process have been reported. In this paper the genes encoding enzymes involved in carbohydrate metabolism including Calvin cycle, sucrose synthesis and degradation, starch synthesis and degradation and genes encoding sugar transporters were analyzed systematically. 148 genes encoding carbohydrate metabolism enzymes and 102 genes encoding sugar transporters were identified. 228 of these genes have been reported previously and 22 genes were found for the first time. Expression profile analysis indicates that some genes showed tissue specific expression pattern, which is closely related to their function. Expression of many genes was affected by abiotic stresses, demonstrating the important role of these genes in resistance to stress.

Key words: rice（Oryza sativa L.）, carbohydrate metabolism, sugar transporter, yield formation

中图分类号:

S511
Q78

王义杰, 张绍杰, 赖艳, 胡永峰. 水稻糖代谢相关酶和糖类转运蛋白编码基因的鉴定和表达分析[J]. 湖北农业科学, 2019, 58(22): 185-193.

WANG Yi-jie, ZHANG Shao-jie, LAI Yan, HU Yong-feng. Identification and expression analysis of genes encoding carbohydrate metabolism enzymes and sugar transporters[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(22): 185-193.

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水稻糖代谢相关酶和糖类转运蛋白编码基因的鉴定和表达分析

Identification and expression analysis of genes encoding carbohydrate metabolism enzymes and sugar transporters

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