湖北农业科学 ›› 2019, Vol. 58 ›› Issue (23): 63-71.doi: 10.14088/j.cnki.issn0439-8114.2019.23.016

• 资源·环境 • 上一篇    下一篇

淹水胁迫对小白菜幼苗地上部生长和生理特性的影响

朱进1, 彭玉全1, 沈若妍1, 李文静1, 周国林2, 黄兴学2   

  1. 1.长江大学湿地生态与农业利用教育部工程研究中心/长江大学园艺园林学院,湖北 荆州 434025;
    2.武汉市农业科学院蔬菜研究所,武汉 430065
  • 收稿日期:2019-07-11 出版日期:2019-12-10 发布日期:2019-12-18
  • 通讯作者: 黄兴学,主要从事蔬菜栽培与生理生态研究,(电话)18986271411(电子信箱)hxx329517@126.com。
  • 作者简介:朱 进(1969-),男,湖北仙桃人,教授,博士,主要从事设施蔬菜栽培与逆境生理方面的研究,(电话)13697194518(电子信箱) 278162100@qq.com
  • 基金资助:
    长江大学湿地生态与农业利用教育部工程研究中心开放基金项目(KF201803); 武汉市农业科学院开放性课题(KFxkt201801)

Effects of flooding stress on growth and physiological characteristics of Chinese cabbage seedlings

ZHU Jin1, PENG Yu-quan1, SHEN Ruo-yan1, LI Wen-jing1, ZHOU Guo-lin2, HUANG Xing-xue2   

  1. 1.Engineering Research Center of Ecology and Agricultural Use of Wetland,Ministry of Education/College of Horticulture and Gardening,Yangtze University,Jingzhou 434025,Hubei,China;
    2.Vegetable Research Institute of Wuhan Academy of Agricultural Sciences,Wuhan 430065,China
  • Received:2019-07-11 Online:2019-12-10 Published:2019-12-18

摘要: 采用双因素随机区组设计研究了淹水胁迫对小白菜(Brassicacampestris ssp.chinensis L.)幼苗地上部生长和生理特性的影响。结果表明,淹水10、15 d时,4个小白菜品种的地上部鲜重和干重都显著低于对照,说明淹水胁迫抑制了小白菜地上部的生长,但4个小白菜品种在淹水15 d时都没有死亡,说明4个小白菜品种都比较耐淹。淹水5、10和15 d时,4个小白菜品种的叶绿素含量都显著低于对照,丙二醛含量都显著高于对照,说明在淹水胁迫下,小白菜的光合作用被抑制,受到了较高程度的伤害。在淹水10和15 d,4个小白菜品种的SOD、POD、CAT、PPO、APX活性和可溶性糖、可溶性蛋白质、脯氨酸含量都显著高于对照,说明4个小白菜品种一方面通过提高抗氧化酶活性来抵抗淹水胁迫,另一方面通过增加渗透调节物质含量来适应淹水胁迫。恢复5 d后,4个小白菜品种的CAT活性、丙二醛和脯氨酸含量都比淹水15 d时显著降低,说明小白菜的生理状态有所恢复。

关键词: 淹水胁迫, 小白菜(Brassicacampestris ssp.chinensis L.)幼苗, 生长发育, 生理特性

Abstract: The effects of flooding stress on aboveground growth and physiological characteristics of Chinese cabbage (Brassicacampestris ssp.chinensis L.) seedlings were studied by using a two-factor randomized block design. The results showed that the above ground fresh weight and dry weight of four Chinese cabbage varieties were significantly lower than the control at 10 and 15 days after waterlogging, indicating that the above ground growth of Chinese cabbage was inhibited by waterlogging stress. However, the four Chinese cabbage varieties did not die after 15 days of flooding, indicating that the four Chinese cabbage varieties were relatively resistant to flooding. At 5 days, 10 days and 15 days after waterlogging, the chlorophyll content of the four Chinese cabbage varieties were significantly lower than that of the control, and the malondialdehyde content was significantly higher than that of the control, indicating that the photosynthesis of Chinese cabbage was inhibited under waterlogging stress and was damaged to a higher degree. Meanwhile, the activity of SOD, POD, CAT, PPO, APX and the content of soluble sugar, soluble protein and proline of the four Chinese cabbage varieties were significantly higher than that of the control group at 10 and 15 days after waterlogging, indicating that the four Chinese cabbage varieties could resist waterlogging stress by improving the activity of antioxidant enzymes on the one hand, and adapt to waterlogging stress by increasing the content of osmotic regulatory substances on the other hand. After 5 days of recovery, the CAT activity, MDA and proline contents of the 4 Chinese cabbage varieties were significantly lower than that after 15 days of flooding, indicating that the physiological state of Chinese cabbage was restored to some extent.

Key words: waterlogging stress, Chinese cabbage (Brassicacampestris ssp.chinensis L.) seedling, growth, physiological characteristics

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