湖北农业科学 ›› 2019, Vol. 58 ›› Issue (24): 136-141.doi: 10.14088/j.cnki.issn0439-8114.2019.24.033

• 园艺·特产 • 上一篇    下一篇

干旱胁迫对杜鹃叶片超微结构的影响

周媛1, 童俊1, 毛静1, 徐冬云1, 方林川1, 董艳芳1, 刘义满1, 郭彩霞2, 谭庆2, 杨俊娟3, 祝安新3   

  1. 1.武汉市农业科学院林业果树研究所/湖北省园林植物工程技术中心,武汉 430075;
    2.武汉市农业科学院,武汉 430065;
    3.武汉市江夏区林业科学技术推广站,武汉 430000
  • 收稿日期:2019-08-12 出版日期:2019-12-25 发布日期:2019-12-25
  • 作者简介:周 媛(1981-),女,湖北武汉人,高级工程师,博士,主要从事园林植物栽培生理与育种研究,(电话)027-87518860(电子信箱)zhouyuan@wuhanagri.com。
  • 基金资助:
    武汉市农业科学院2019年创新项目(CX201904; CXJC201901)

Effect of drought stress on leaf ultrastructure of Rhododendron L.

ZHOU Yuan1, TONG Jun1, MAO Jing1, XU Dong-yun1, FANG Lin-chuan1, DONG Yan-fang1, LIU Yi-man1, GUO Cai-xia2, TAN Qing2, YANG Jun-juan3, ZHU An-xin3   

  1. 1.Institute of Forestry & Fruit-tree,Wuhan Academy of Agricultural Sciences/Hubei Engineering Technology Research Center of Landscape Plant,Wuhan 430075,China;
    2.Wuhan Academy of Agricultural Sciences,Wuhan 430065,China;
    3.Jiangxia Forestry Science and Technology Promotion Station,Wuhan 430000,China
  • Received:2019-08-12 Online:2019-12-25 Published:2019-12-25

摘要: 以胭脂蜜(Rhododendron obtusum cv. Yanzhimi)、花蝴蝶(Rhododendron obtusum cv. Huahudie)和西施(Rhododendron obtusum cv. Xishi)3个杜鹃品种为研究试材,在光照培养箱内采用盆栽控水法进行干旱胁迫处理,利用透射电镜观测叶片细胞超微结构。结果表明,杜鹃叶片叶绿体和线粒体都随干旱胁迫的程度加深而受到损坏,叶绿体对于水分的亏缺较线粒体更加敏感。对于抗旱性较强的胭脂蜜,质壁分离出现更晚,叶绿体和线粒体的损伤程度更轻,叶片细胞结构对于杜鹃抗旱性强弱的决定作用较大。

关键词: 杜鹃, 干旱胁迫, 抗旱性, 超微结构, 透射电镜

Abstract: Using transmission electron microscope, the leaf ultrastructure were investigated in Rhododendron obtusum cv. Yanzhimi, Rhododendron obtusum cv. Huahudie and Rhododendron obtusum cv. Xishi under drought stress in light incubator. The results showed that the damage of mitochondria and chloroplast increased as the drought degree increased, and chloroplast was more sensitive than mitochondria to the external environment. The plasmolysis appeared later in Rhododendron obtusum ‘Yanzhimi’ with relatively highest drought resistance. In which, the chloroplast and mitochondria were less damaged. There was an important association between leaf cell structure and drought resistance in Rhododendron L..

Key words: Rhododendron L., drought stress, drought resistance, ultrastructure, transmission electron microscope (TEM)

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