湖北农业科学 ›› 2020, Vol. 59 ›› Issue (5): 88-91.doi: 10.14088/j.cnki.issn0439-8114.2020.05.017

• 园艺·特产 • 上一篇    下一篇

不同烤烟品种烟叶氨基酸代谢差异分析

李洪臣1, 朱顺成2   

  1. 1河南省烟草公司三门峡市公司,河南 三门峡 472000;
    2三门峡市烟草公司卢氏县分公司,河南 三门峡 472000
  • 修回日期:2019-09-23 出版日期:2020-03-10 发布日期:2020-06-05
  • 通讯作者: 朱顺成(1969-),男,河南三门峡人,主要从事烟叶生产技术研究工作,(电话)13839885690(电子信箱)895962290@qq.com。
  • 作者简介:李洪臣(1985-),男,河北唐山人,硕士,主要从事烟草品种选育与推广工作,(电话)15138178855(电子信箱)365451255@qq.com
  • 基金资助:
    河南省烟草公司转型升级科技攻关项目(HYKJ201801)

Analysis of amino acid metabolism in tobacco leaves among different varieties

LI Hong-chen1, ZHU Shun-cheng2   

  1. 1.Sanmenxia Company of Henan Tobacco Company,Sanmenxia 472000,Henan,China;
    2.Lushi Company of Sanmenxia Tobacco Company,Sanmenxia 472000,Henan,China
  • Revised:2019-09-23 Online:2020-03-10 Published:2020-06-05

摘要: 为筛选优质烤烟品种,明确烤烟品种间烟叶氨基酸代谢差异情况,研究了豫烟10号、NC89和中烟100 3个品种烟叶生长过程中脯氨酸、谷氨酸和天冬氨酸代谢关键酶活性及氨基酸含量动态变化。结果表明,叶龄20 d时,Δ1-吡咯啉-5-羧酸合成酶、鸟氨酸转氨酶、脯氨酸脱氢酶、天冬氨酸转氨酶和天冬氨酸激酶活性均呈先升高后降低趋势,峰值在叶龄30~40 d,豫烟10号酶活性最高;烟叶中脯氨酸含量不断增加,叶龄30 d后,豫烟10号脯氨酸含量最高。游离氨基酸含量呈先升高后降低再升高趋势,在叶龄各时期豫烟10号均最高。

关键词: 烤烟, 烟叶, 氨基酸, 酶活性

Abstract: : To screen high-quality flue-cured tobacco varieties and clarify the differences in amino acid metabolism among flue-cured tobacco varieties, the dynamic changes of proline, glutamic acid and aspartic acid metabolism key enzyme activities and amino acid content during the tobacco leaf growth of Yuyan 10, NC89 and Zhongyan 100 were studied. The results showed that at 20 days of leaf age, the activities of Δ1-pyrroline-5-carboxylic acid synthetase, ornithine aminotransferase, proline dehydrogenase, aspartate aminotransferase and aspartate kinase all increased first and then decreased, the peak is at leaf age 30~40 d, Yuyan 10 had the highest enzyme activity; the content of proline in tobacco leaves increased continuously; after 30 days of leaf age, the content of proline in Yuyan 10 changed to highest. The content of free amino acids increased first, then decreased and then increased, and Yuyan 10 was the highest at each stage of leaf age.

Key words: flue-cured tobacco, tobacco leaf, amino acid, enzyme activity

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