不同浓度6-BA对变异株云烟97继代培养的影响

doi:10.14088/j.cnki.issn0439-8114.2021.02.020

湖北农业科学 ›› 2021, Vol. 60 ›› Issue (2): 100-103.doi: 10.14088/j.cnki.issn0439-8114.2021.02.020

不同浓度6-BA对变异株云烟97继代培养的影响

张丽琼, 陈迪, 杨松杰, 聂祥涛

安康学院现代农业与生物科技学院,陕西安康 725000

收稿日期:2020-04-14 出版日期:2021-01-25 发布日期:2021-02-07
作者简介:张丽琼（1979-）,女,云南个旧人,副教授,博士,主要从事土壤物质循环研究,（电话）13909158066（电子信箱）yryzlq@163.com。
基金资助:
陕西省大学生创新创业训练计划项目（2017sxjy002）

Effects of different concentrations of 6-BA on the secondary culture of variant Yunyan 97

ZHANG Li-qiong, CHEN Di, YANG Song-jie, NIE Xiang-tao

School of Modern Agriculture and Biotechnology, Ankang University, Ankang 725000, Shaanxi, China

Received:2020-04-14 Online:2021-01-25 Published:2021-02-07

摘要/Abstract

摘要： 在陕西省安康市镇坪县发现2株云烟97巨型变异株,比普通植株有较大的生长优势,利用组培快繁的方式保留这一优良种质资源,以云烟97变异株的腋芽诱导的组培苗为原材料,研究在不同培养基配方下不同6-BA浓度对变异株云烟97继代培养的影响。结果表明,云烟97变异株适宜的分化培养基为二代培养基MS+6-BA 2.0 mg/L,三代培养基MS+6-BA 1.0 mg/L+NAA 0.2 mg/L,在此条件下,变异株分化成芽率较高,达到88.9%,

关键词: 云烟97, 变异株, 快速繁殖, 继代培养

Abstract: Two giant variants of Yunyan 97 were found in Zhenping county, Ankang city, Shaanxi province,compared with the common tobacco, these giant variants have larger growth advantage. It is a good germplasm resource to be preserved by tissue culture and rapid propagation. The effects of 6-BA concentration on the secondary culture of Yunyan 97 were studied by using tissue culture seedlings induced by axillary buds of Yunyan 97 variant as raw materials. The results showed that the suitable differentiation medium for Yunyan 97 variants was MS+6-BA 2.0 mg/L, and the third generation medium MS+6-BA 1.0 mg/L+NAA 0.2 mg/L, under these conditions, the differentiation rate of variant was 88.9%.

Key words: Yunyan 97, variant, rapid propagation, blasting rate, secondary culture

中图分类号:

S572

张丽琼, 陈迪, 杨松杰, 聂祥涛. 不同浓度6-BA对变异株云烟97继代培养的影响[J]. 湖北农业科学, 2021, 60(2): 100-103.

ZHANG Li-qiong, CHEN Di, YANG Song-jie, NIE Xiang-tao. Effects of different concentrations of 6-BA on the secondary culture of variant Yunyan 97[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(2): 100-103.

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不同浓度6-BA对变异株云烟97继代培养的影响

Effects of different concentrations of 6-BA on the secondary culture of variant Yunyan 97

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