湖北农业科学 ›› 2021, Vol. 60 ›› Issue (12): 81-84.doi: 10.14088/j.cnki.issn0439-8114.2021.12.016

• 园艺·特产 • 上一篇    下一篇

PEG模拟干旱胁迫对不同桑树砧木生理生化特性的影响

张朝华1, 张娜2, 朱琳琳3, 莫荣利2, 于翠2   

  1. 1.广西壮族自治区蚕业技术推广站,南宁 530007;
    2.湖北省农业科学院经济作物研究所,武汉 430064;
    3.南漳县农业科学研究所,湖北 南漳 441500
  • 收稿日期:2020-03-29 出版日期:2021-06-25 发布日期:2021-07-19
  • 通讯作者: 莫荣利,助理研究员,博士,主要从事果桑育种与栽培研究,(电子信箱)moqianchun1987@163.com;于 翠,研究员,硕士生导师,主要从事果桑育种与栽培研究,(电子信箱)mrsyu888@hotmail.com。
  • 作者简介:张朝华(1980-),男,广西桂林人,农艺师,主要从事桑树种质资源收集、评价研究,(电话)13481135759(电子信箱)28357738@qq.com。
  • 基金资助:
    湖北省农业科学院青年基金项目(2017NKYJJ07); 湖北省技术创新专项重大项目(2019ABA090); 国家重点研发计划项目(2019YFD1000600); 湖北省自然科学基金重点类项目(2020CFA061); 现代农业产业技术体系建设专项(CARS-18)

Effects of PEG-simulated drought stress on physiological and biochemical characteristics of different mulberry rootstocks

ZHANG Chao-hua1, ZHANG Na2, ZHU Lin-lin3, MO Rong-li2, YU Cui2   

  1. 1. Sericulture Technology Promotion Station of Guanxi Zhuang Autonomous Region,Nanning 530007,China;
    2. Institute of Economic Crops,Hubei Academy of Agricultural Sciences,Wuhan 430064,China;
    3. Institute of Nanzhang County Agricultural Science,Nanzhang 441500,Hubei,China
  • Received:2020-03-29 Online:2021-06-25 Published:2021-07-19

摘要: 采用5%的PEG模拟重度干旱胁迫研究不同桑树砧木的生理生化特性。在重度干旱胁迫下,桑树砧木叶片迅速失去水分,不同砧木的萎焉时间和萎焉率有明显差异。丙二醛(MDA)、脯氨酸(Pro)、过氧化氢(H2O2)含量、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、过氧化氢酶(CAT)活性等与抗逆性相关的生理生化指标的测定结果显示,6个砧木的MDA含量、SOD活性、CAT活性、POD活性总体呈下降趋势,但变化幅度不明显,而Pro含量呈升高的趋势,尤其是后期Pro含量迅速增加,H2O2含量呈先升高后降低的变化特点,但在不同砧木间的变化趋势存在差异。

关键词: 桑树, 砧木, 干旱胁迫

Abstract: In this study, 5% PEG was used to simulate severe drought stress to explore the physiological and biochemical characteristics of different mulberry rootstocks. Under severe drought stress, the leaves of mulberry rootstocks quickly lose water, and the wilting time and wilting ratio of different rootstocks are significantly different. Physiological and biochemical indicators related to water stress, such as malondialdehyde (MDA), proline (Pro), hydrogen peroxide (H2O2) content, and the activities of superoxide dismutase (SOD) activity, peroxidase (POD) activity, catalase (CAT) activity were measured. The results of the study showed that the MDA content, SOD activity, CAT activity, and POD activity of the six mulberry rootstocks showed an overall downward trend, but the magnitude of the change was slight. However, the Pro content showed an increasing trend, especially in the later period, the Pro content increased rapidly, and the content of H2O2 showed the characteristic of first increasing and then decreasing, but the trend of change between different mulberry rootstocks were different.

Key words: mulberry, rootstock, drought stress

中图分类号: