甘蓝型油菜花粉粒ARTP诱变及后代表型鉴定

doi:10.14088/j.cnki.issn0439-8114.2021.19.004

湖北农业科学 ›› 2021, Vol. 60 ›› Issue (19): 19-22.doi: 10.14088/j.cnki.issn0439-8114.2021.19.004

甘蓝型油菜花粉粒ARTP诱变及后代表型鉴定

何晓莹¹, 陆文虎¹, 师伟策¹, 王文敏², 王敬乔¹, 陈苇¹, 张国建³, 张建昆³, 束正齐¹, 俎峰¹

1.云南省农业科学院经济作物研究所,昆明 650205;
2.昆明深洪生物科技开发有限公司,昆明 650051;
3.云南省农业职业技术学院,昆明 650031

收稿日期:2020-12-07 出版日期:2021-10-10 发布日期:2021-10-22
通讯作者: 俎峰（1984-）,男,河南许昌人,副研究员,硕士,主要从事油菜种质创新与分子育种工作,（电子信箱）zufeng1984@163.com。
作者简介:何晓莹（1986-）,女,云南丽江人,助理研究员,硕士,主要从事油菜分子标记辅助育种工作,（电话）13708841815（电子信箱）250911760@qq.com。
基金资助:
国家自然科学基金项目（31760394）; 云南省农业基础研究联合专项（2017FG001-070）; 云南省农业科学院食用油料作物育种与应用研究省创新团队（2017HC021）

ARTP irradiation on pollen grain of Brassica napus and phenotype identification of their offsprings

HE Xiao-ying¹, LU Wen-hu¹, SHI Wei-ce¹, WANG Wen-min², WANG Jing-qiao¹, CHEN Wei¹, ZHANG Guo-jian³, ZHANG Jian-kun³, SHU Zheng-qi¹, ZU Feng¹

1. Industrial Crops Research Institute,Yunnan Academy of Agricultural Sciences,Kunming 650205,China;
2. Kunming Shenhong Biotechnology Development Co. ,Ltd. , Kunming 650051,China;
3. Yunnan Vocational and Technical College of Agriculture,Kunming 650031,China

Received:2020-12-07 Online:2021-10-10 Published:2021-10-22

摘要/Abstract

摘要： 为获得高含油量油菜的变异株系,开展了甘蓝型油菜（Brassica napus L.）花粉粒ARTP诱变试验。以甘蓝型油菜Ogu-CMS恢复系16C花粉为诱变材料,采用ARTP诱变技术分别辐照0、20、30、40 min,再将诱变后的花粉授于同一群体中自然产生的不育单株16CS。调查不同ARTP辐照时间下的诱变花粉授粉当代单角粒数及后代子粒发芽势、发芽率、不育株率和含油量的变化情况。结果表明,ARTP辐照甘蓝型油菜花粉30 min 以上,能显著降低诱变花粉授粉当代单角粒数及后代M₀代子粒的发芽势和发芽率,提升M₀代植株的不育株率,提升M₁代子粒的含油量,获得了含油量高达52.61%突变株系ARTP30-315。初步表明利用ARTP-P仪开展油菜花粉粒诱变是有效的,较为适宜的ARTP辐照时间为30~40 min。为生产上应用ARTP-P仪开展甘蓝型油菜花粉粒诱变探索出一个较为适宜的参数体系,为开展油菜种质创新提供了新的方法与思路。

关键词: 甘蓝型油菜（Brassica napus L.）, Ogu-CMS 恢复系, 花粉粒, ARTP诱变, 雄性不育, 含油量

Abstract: In order to obtain the mutant lines of high oil content rape, the ARTP mutation experiment of pollen grains of Brassica napus was carried out.The pollen of the restoring line 16C of Ogu CMS in Brassica napus was irradiated with ARTP mutation technology for 0, 20, 30 and 40 min respectively, and the mutated pollen was given to the sterile single plant 16CS naturally produced in the same population. Then traits of the progeny, such as seed number per pod, seed germination rate and energy and oil content were surveryed. The results showed that ARTP irradiation of Brassica napus pollen for more than 30 min could significantly reduce the number of single horned grains at the time of mutagenic pollen pollination and the germination potential and germination rate of M₀ generation seeds, improve the sterile plant rate of M₀ generation plants and the oil content of M₁ Generation seeds, and obtain the mutant line ARTP30-315 with an oil content of 52.61%. The preliminary results showed that it was effective to carry out rape pollen mutation by ARTP-P instrument, and the more suitable ARTP irradiation time was 30~40 min. A more suitable parameter system was explored for carrying out pollen mutation of Brassica napus by ARTP-P instrument in production, which provided a new method and idea for carrying out germplasm innovation of Brassica napus in the future.

Key words: Brassica napus L., Ogu-CMS restorer, pollen grain, ARTP mutation, male sterility, oil content

中图分类号:

S335

何晓莹, 陆文虎, 师伟策, 王文敏, 王敬乔, 陈苇, 张国建, 张建昆, 束正齐, 俎峰. 甘蓝型油菜花粉粒ARTP诱变及后代表型鉴定[J]. 湖北农业科学, 2021, 60(19): 19-22.

HE Xiao-ying, LU Wen-hu, SHI Wei-ce, WANG Wen-min, WANG Jing-qiao, CHEN Wei, ZHANG Guo-jian, ZHANG Jian-kun, SHU Zheng-qi, ZU Feng. ARTP irradiation on pollen grain of Brassica napus and phenotype identification of their offsprings[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(19): 19-22.

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甘蓝型油菜花粉粒ARTP诱变及后代表型鉴定

ARTP irradiation on pollen grain of Brassica napus and phenotype identification of their offsprings

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