基于网络药理学和免疫浸润的大蒜素对肝细胞癌作用机制分析

doi:10.14088/j.cnki.issn0439-8114.2022.10.014

湖北农业科学 ›› 2022, Vol. 61 ›› Issue (10): 83-89.doi: 10.14088/j.cnki.issn0439-8114.2022.10.014

基于网络药理学和免疫浸润的大蒜素对肝细胞癌作用机制分析

董雪静, 朱华, 王颖

广西中医药大学科学实验中心,南宁 530200

收稿日期:2021-04-09 出版日期:2022-05-25 发布日期:2022-06-14
作者简介:董雪静（1995-）,女,山东威海人,在读硕士研究生,研究方向为中药及民族药的鉴定与开发,（电话）18776710710（电子信箱）441350041@qq.com;朱华（1957-）,男, 广西柳州人,教授,博士,博士生导师,主要从事中药品种、质量及资源开发研究,（电话）18977165885（电子信箱）zhuhuagx@163.com。
基金资助:
国家自然科学基金项目（82060695）; 壮瑶药协同创新中心研究生科研课题（ZYYDC-06）

The mechanism analysis of allicin in hepatocellular carcinoma based on network pharmacology and immune infiltration

DONG Xue-jing, ZHU Hua, WANG Ying

Science Experiment Center, Guangxi University of Chinese Medicine, Nanning 530200,China

Received:2021-04-09 Online:2022-05-25 Published:2022-06-14

摘要/Abstract

摘要： 利用网络药理学和免疫浸润探讨大蒜素对肝细胞癌的作用机制。利用TCMSP数据库收集大蒜素的有效成分信息,利用PharmMapper数据库筛选大蒜素的作用靶点;通过STRING11.0数据库进行蛋白互作分析,并利用Cytoscape3.7.1软件进行可视化展示。通过GeneCards数据库收集肝细胞癌相关靶点,将化合物靶点与疾病相关靶点进行比对,利用Venny2.1.0工具绘制韦恩图。利用GEPIA数据库对潜在靶点进行表达差异分析和生存预后分析,最后利用TIMER数据库进行肿瘤细胞免疫浸润分析。结果表明,筛选得到大蒜素的潜在靶点有194个,与HCC相关的靶点31个,其中高相关性靶点4个。大蒜素治疗肝细胞癌具有多靶点、多途径、多通路的特点,CCNA2、AURKA、MMP9、ESR1等靶点可能在治疗过程发挥重要作用。

关键词: 大蒜素, 肝细胞癌, 网络药理学, 免疫浸润

Abstract: The mechanism of allicin on hepatocellular carcinoma was explored using network pharmacology and immune infiltration. The effective composition information of allicin was collected by TCMSP database, and PharmMapper database was used to screen the targets of allicin; the above data were imported into STRING11.0 database for PPI network analysis, and then the protein interaction network was visualized by Cytoscape3.7.1 software. Hepatocellular carcinoma related targets were collected through the GeneCards database, then the targets of allicin were compared with the disease-related-targets, and Venny2.1.0 tool was used to draw the Venn diagram. GEPIA database was used to analyze the expression differences and survival prognosis of potential targets, and then TIMER database was used to analyze the immune infiltration of tumor cells. Results showed that 194 potential targets of allicin were screened and 31 targets related to HCC were identified. After screening with P<0.05,4 signal pathways were obtained. The therapeutic effect of allicin on hepatocellular carcinoma reflects the characteristics of multi-target, multi-pathway and multi-component of traditional Chinese medicine. CCNA2, AURKA, MMP9, ESR1 and other targets may play an important role in the treatment process.

Key words: allicin, hepatocellular carcinoma, network pharmacology, immune infiltration

中图分类号:

R285

董雪静, 朱华, 王颖. 基于网络药理学和免疫浸润的大蒜素对肝细胞癌作用机制分析[J]. 湖北农业科学, 2022, 61(10): 83-89.

DONG Xue-jing, ZHU Hua, WANG Ying. The mechanism analysis of allicin in hepatocellular carcinoma based on network pharmacology and immune infiltration[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(10): 83-89.

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基于网络药理学和免疫浸润的大蒜素对肝细胞癌作用机制分析

The mechanism analysis of allicin in hepatocellular carcinoma based on network pharmacology and immune infiltration

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