湖北农业科学 ›› 2021, Vol. 60 ›› Issue (2): 109-115.doi: 10.14088/j.cnki.issn0439-8114.2021.02.022

• 园艺·特产 • 上一篇    下一篇

网络药理学方法研究三七治疗关节炎的作用机制

韩倩1, 陈勇1, 王竟静1, 魏江存2, 黄周艳1, 陈良妮1   

  1. 1.广西中医药大学科学实验中心/药学院,南宁 530200;
    2.广西国际壮医医院,南宁 530201
  • 收稿日期:2020-04-24 出版日期:2021-01-25 发布日期:2021-02-07
  • 通讯作者: 陈 勇(1961-),男,教授,硕士研究生导师,主要从事中药及其制剂质量分析研究,(电子信箱)cy6381@163.com。
  • 作者简介:韩 倩(1996-),女,广西宜州人,在读硕士研究生,研究方向为药物质量控制研究,(电话)18777848879(电子信箱)1648997811@qq.com。
  • 基金资助:
    国家自然科学基金项目(81660701); 广西自然科学基金项目(2016GXNSFAA380148); 壮瑶药协同创新中心项目(桂教科研〔2013〕20号); 广西壮瑶药重点实验室(桂科基字〔2014〕32号); 广西重点学科壮药学(桂教科研〔2013〕16号); 广西八桂学者中药创新理论与药效研究(J13162); 广西中药药效研究重点实验室(17-259-20); 广西高校中药提取纯化与质量分析重点实验室(桂教科研〔2014〕6号); 国家中医药管理局科研三级实验室中(壮)药化学与质量分析实验室(国中医药发2009[21]号); 广西中医药大学研究生教育创新计划项目(YCSY20200015)

Mechanism of Panax notoginseng in the treatment of arthritis based on network pharmacology

HAN Qian1, CHEN Yong1, WANG Jing-jing1, WEI Jiang-cun2, HUANG Zhou-yan1, CHEN Liang-ni1   

  1. 1. Scientific Experiment Center/College of Pharmacy,Guangxi University of Chinese Medicine, Nanning 530200,China;
    2. Guangxi International Hospital of Zhuang Medicine,Nanning 530201,China
  • Received:2020-04-24 Online:2021-01-25 Published:2021-02-07

摘要: 运用中药系统药理学分析平台(TCMSP)数据库,以化合物口服利用度(OB)>30%、药物相似性(DL)>0.18为条件,检索得到三七(Panax notoginseng)的主要有效成分;通过Drugbank、UniProt数据库进行活性成分靶点提取并用GeneCards、OMIM数据库筛选出关节炎的相关靶点,制作三七治疗关节炎的相关靶点的Veen图,进而使用Cytoscape 3.6.0软件、String数据库分别绘制三七-有效活性成分-靶基因-关节炎的中药网络调控图和蛋白互作(PPI)网络图,最后以R语言为工具,筛选关键靶点并分析其生物过程和重要信号通路,利用网络药理学方法探究三七治疗关节炎的作用机制。结果表明,检索到三七的8个主要成分,得到β-谷甾醇、豆甾醇、人参皂苷rh2等;预测三七治疗关节炎的相关靶点131个;三七关键靶点主要涉及蛋白质异二聚活性、DNA结合转录激活活性,RNA聚合酶II特异性、细胞因子受体结合等生物过程;主要通过糖尿病并发症AGE-RAGE信号通路、流体剪切应力与动脉粥样硬化、乙型肝炎等重要信号通路发挥治疗关节炎的作用。

关键词: 三七(Panax notoginseng), 关节炎, 网络药理学

Abstract: The main effective components of Panax notoginseng were retrieved by TCMSP database under the condition of OB > 30% and DL > 0.18; The target of active ingredients was extracted by Drugbank and UniProt database, and the related targets of arthritis were screened by GeneCards and OMIM database;making Veen map of related targets of Panax notoginseng in the treatment of arthritis; Then, we use the software of Cytoscape 3.6.0 and the database of String to draw PPI network diagram and the network control diagram of Panax notoginseng, active ingredient, target gene and arthritis respectively; Finally, R language is used as a tool to screen key targets and analyze their biological processes and important signaling pathways, to study the mechanism of Panax notoginseng in the treatment of arthritis by network pharmacology. The results showed that eight main components of Panax notoginseng were retrieved, including β-sitosterol, stigmasterol and ginsenoside rh2; 131 related targets of Panax notoginseng in the treatment of arthritis; The key targets of Panax notoginseng are mainly involved in protein heterodimerization activity, DNA-binding transcription activator activity,RNA polymerase II-specific,cytokine receptor binding and other biological processes; It is necessary to play a role in the treatment of arthritis through AGE-RAGE signaling pathway in diabetic complication,fluid shear stress and atherosclerosis, Hepatitis B and other important signal pathways.

Key words: Panax notoginseng, arthritis, network pharmacology

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