健脾益气口服液对小鼠小肠推进功能的影响及其作用机制分析

doi:10.14088/j.cnki.issn0439-8114.2021.17.021

湖北农业科学 ›› 2021, Vol. 60 ›› Issue (17): 98-103.doi: 10.14088/j.cnki.issn0439-8114.2021.17.021

健脾益气口服液对小鼠小肠推进功能的影响及其作用机制分析

刘巧明¹, 谢胜², 奉建芳¹, 王力宁², 陈雅璐², 林国彪¹, 黄晓燕², 梁健钦¹

1.广西中医药大学,南宁 530001;
2.广西中医药大学第一附属医院,南宁 530023

收稿日期:2020-11-16 出版日期:2021-09-10 发布日期:2021-09-30
通讯作者: 黄晓燕（1979-）,女,副主任医师,博士,主要从事中医药防治消化系统疾病的研究工作,（电话）18275785900（电子信箱）huangxiaoyan0705@163.com。
作者简介:刘巧明（1995-）,女,广西贵港人,在读硕士研究生,研究方向为新药新产品的开发,（电话）17754559206（电子信箱）1348156551@qq.com
基金资助:
广西创新驱动发展专项（桂科AA17202031）

Effect of Jianpi Yiqi Koufuye on small intestine propulsion in mice and analysis of its mechanism

LIU Qiao-ming¹, XIE Sheng², FENG Jian-fang¹, WANG Li-ning², CHEN Ya-lu², LIN Guo-biao¹, HUANG Xiao-yan², LIANG Jian-qin¹

1. Guangxi University of Traditional Chinese Medicine,Nanning 530001,China;
2. The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine,Nanning 530023,China

Received:2020-11-16 Online:2021-09-10 Published:2021-09-30

摘要/Abstract

摘要： 探讨健脾益气口服液对功能性消化不良的影响及其作用机制。取KM小鼠60只,雌雄各半,随机分为空白组、模型组、健脾益气口服液（高、中、低、极低剂量）组,灌胃给药4周,采用墨汁法测定小肠推进率。通过GeneCards、Drugbank数据库筛选疾病靶点。通过BATMAN-TCM搜索处方中药材的化学成分及其靶点。通过String数据库和Cytoscape软件构建靶点互作网络,提取关键靶点,进行GO、KEGG富集分析和网络可视化分析。通过Discovery Studio虚拟筛选与药效相关的化合物、靶点,分析其结合模式。健脾益气口服液组对小鼠小肠推进率与模型组比较有显著提高（P<0.01）。处方中共筛选得到194个化学成分以及703个化学成分作用靶点。功能性消化不良的疾病靶点有601个。从靶点网络中提取得到包含TNF、INS、MAPK1等104个靶点组成的关键网络,参与调节肽激素的分泌、蛋白质磷酸化等生物过程,还参与调控TNF信号通路、IL-17信号通路、cAMP信号通路、cGMP-PKG、Ca²⁺信号通路等。分子对接结果显示,在194个化学成分中,党参、鸡内金所含的烟酸、苯丙氨酸与Ca²⁺信号通路、cAMP信号通路中FOS、EGFR、CREB1、NOS3蛋白以氢键、范德华力等多种分子间作用力结合。健脾益气口服液促进小鼠小肠的推进功能,其机制可能与烟酸、苯丙氨酸等参与调控cAMP信号通路、Ca²⁺信号通路中FOS、EGFR、CREB1、NOS3的蛋白表达有关。健脾益气口服液可用于治疗功能性消化不良。

关键词: 健脾益气口服液, 功能性消化不良, 小肠推进, 网络药理学, 分子对接

Abstract: To investigate the effects of Jianpi Yiqi Koufuye on functional dyspepsia and its mechanism of action. Sixty KM mice, half male and half female, were randomly divided into blank group, model group, and Jianpi Yiqi Koufuye （high, medium, low, and very low dose） group, administered orally for 4 weeks, and determined by the ink method small bowel advancing rate. Screen disease targets through GeneCards and Drugbank databases. Search BATMAN-TCM for the chemical constituents and their targets in Chinese herbal medicines. Through the String database and Cytoscape software, a target interaction network was constructed, key targets were extracted, and GO, KEGG enrichment analysis, and network visualization analysis were performed. Virtually screen compounds and targets related to drug efficacy through Discovery Studio and analyze their binding patterns. The Jianpi Yiqi Koufuye group significantly improved the small bowel advancing rate of the mice compared with the model group （P<0.01）. A total of 194 chemical constituents and 703 chemical constituent targets were screened from the prescription. There were 601 targets for functional dyspepsia.A key network consisting of 104 targets, including TNF, INS and MAPK1, was extracted from the target network, which was involved in the regulation of the secretion of peptide hormones, protein phosphorylation and other biological processes, as well as the regulation of TNF signaling pathway, IL-17 signaling pathway, cAMP signaling pathway, cGMP-PKG and Ca²⁺ signaling pathway. Molecular docking results showed that in 194 chemical components, niacin and phenylalanine contained in codonopsis codonopsis and chicken gold bound with FOS, EGFR, CREB1 and NOS3 proteins in Ca²⁺ signaling pathway and cAMP signaling pathway by hydrogen bond and van der Waals force. Jianpi Yiqi Koufuye promotes the small intestine's advancing function in mice, and its mechanism may be related to the expression of FOS, EGFR, CREB1, and NOS3 in the cAMP signaling pathway and Ca²⁺ signaling pathway, which are involved in the regulation of nicotinic acid and phenylalanine. Jianpi Yiqi Koufuye can be used to treat functional indigestion.

Key words: Jianpi Yiqi Koufuye, functional dyspepsia, small intestinal propulsion, network pharmacology, molecular docking

中图分类号:

R285.5

刘巧明, 谢胜, 奉建芳, 王力宁, 陈雅璐, 林国彪, 黄晓燕, 梁健钦. 健脾益气口服液对小鼠小肠推进功能的影响及其作用机制分析[J]. 湖北农业科学, 2021, 60(17): 98-103.

LIU Qiao-ming, XIE Sheng, FENG Jian-fang, WANG Li-ning, CHEN Ya-lu, LIN Guo-biao, HUANG Xiao-yan, LIANG Jian-qin. Effect of Jianpi Yiqi Koufuye on small intestine propulsion in mice and analysis of its mechanism[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(17): 98-103.

参考文献

[1] 金婷婷. 功能性消化不良的中西医研究概况[A].中国中西医结合学会消化系统疾病专业委员会.第三十一届全国中西医结合消化系统疾病学术会议论文集[C].中国中西医结合学会消化系统疾病专业委员会:中国中西医结合学会,2019.159.
[2] JUNG H O, KWON J G, JUNG H K, et al.Clinical practice guidelines for functional dyspepsia in korea[J]. J Neurogastroenterol Motil, 2020, 26(1): 29-50.
[3] ZHANG J Q, WANG X, SHI X S, et al.Combination of 15 lipid metabolites and motilin to diagnose spleen-deficiency FD[J].Chin Med,2019, 14:16.
[4] 唐燕, 唐梅文, 楼茜欣, 等.功能性消化不良的中医治疗进展[J].中医学报, 2020, 35(2): 1-5.
[5] 叶美玉. 党参山楂健脾养胃咀嚼片的制剂工艺及药效学研究[D].兰州: 兰州大学, 2019.
[6] 王珊珊, 黄楚龙, 黎芳, 等.复方熊胆滴眼液治疗急性细菌性结膜炎作用及其机制的网络药理学分析[J].广东药科大学学报, 2020, 36(1): 78-83.
[7] WU M N, LI Y R, GU Y F.Hesperidin improves colonic motility in Loeramide-Induced constipation rat model via 5-Hydroxytryptamine 4R/cAMP signaling pathway[J].Digestion,2020,101(6):692-705.
[8] 张佳琪, 张丽颖, 王雪, 等.从线粒体功能异常探讨功能性消化不良胃肠动力障碍的发病机制[J].中华中医药杂志,2019,34(8): 3637-3639.
[9] 吕林, 王凤云, 唐旭东, 等.脾虚一号方对脾虚型功能性消化不良大鼠胃组织线粒体呼吸链复合物Ⅳ亚单位的影响[J].中国实验方剂学杂志, 2017, 23(7): 102-108.
[10] LU Y Y, ZHANG Z L, XI C, et al.Study of gastrointestinal tract viability and motility via modulation of serotonin in a zebrafish model by probiotics[J].Food & amp; function, 2019, 10(11): 7416-7425.
[11] 邢德刚. C-型钠尿肽对胆碱能系统调节胃运动作用的影响及其离子通道机制[D].吉林延吉: 延边大学, 2006.
[12] KATE N, NATASHA K, MARJORIE M W, et al.Women and functional dyspepsia[J].Womens health (lond), 2016, 12(2): 241-250.
[13] MOUNIKA A, VICTORIA E D W, SAVIO R, et al. Immunopathological and molecular basis of functional dyspepsia and current therapeutic approaches[J].Expert Rev Clin Immunol,2018, 14(10): 831-840.
[14] CHOI Y J, PARK Y S, KIM N, et al.Gender differences in ghrelin, nociception genes, psychological factors and quality of life in functional dyspepsia[J].World J Gastroenterol, 2017, 23(45): 8053-8061.
[15] 郝志, 刘欢, 乔明琦. 雌激素及其受体介导的5-羟色胺能系统在情绪调控中的作用研究进展[J].中国医药导报, 2016, 13(23): 45-48.
[16] 龙茜, 刘乐平, 郭璇, 等.基于网络药理学探讨柴胡-黄芩药对治疗功能性消化不良的作用机制[J].时珍国医国药, 2019, 30(4): 815-819.
[17] JOHN M R, JOSE T C, JENNIFER V S, et al.Visceral hypersensitivity and electromechanical dysfunction as therapeutic targets in pediatric functional dyspepsia[J]. World J Gastrointest Pharmacol Ther, 2014, 5(3):122-138.
[18] MIN J L, HYE-KYUNG J, KO E L, et al.Degranulated eosinophils contain more fine nerve fibers in the duodenal mucosa of patients with functional dyspepsia[J].J Neurogastroenterol Motil, 2019, 25(2): 212-221.
[19] DENTON R M.Regulation of mitochondrial dehydrogenases by calcium ions[J].Biochimica et biophysica acta,2009,1787(11): 1309-1316.
[20] RAFFAELLO A, MAMMUCARI C, GHERARDI G, et al.Calcium at the center of cell signaling:Interplay between endoplasmic reticulum, mitochondria, and lysosomes[J].Trends in biochemical sciences, 2016, 41(12):103.

健脾益气口服液对小鼠小肠推进功能的影响及其作用机制分析

Effect of Jianpi Yiqi Koufuye on small intestine propulsion in mice and analysis of its mechanism

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 11

编辑推荐

Metrics

本文评价

[1]	徐冬月, 赵声兰. 基于网络药理学及分子对接技术分析核桃治疗高尿酸血症的作用机制[J]. 湖北农业科学, 2023, 62(8): 120-126.
[2]	陈睿, 潘小芳, 杨楠, 霍丽妮, 陈姎玲, 梁天坚. 基于GC-MS和网络药理学探究茉莉花根挥发油镇静安神作用[J]. 湖北农业科学, 2023, 62(11): 84-91.
[3]	陈姎玲, 潘小芳, 徐浩, 陈睿, 霍丽妮, 梁燕. 基于网络药理学及分子对接探讨灵芝药理机制[J]. 湖北农业科学, 2023, 62(10): 83-90.
[4]	柯昌虎, 严慧, 赵阳, 朱军, 李志浩. 基于网络药理学和分子对接探讨黄精抗衰老的作用机制[J]. 湖北农业科学, 2023, 62(10): 100-108.
[5]	曾格格, 刘毅, 刘天琪, 黄振阳, 丁晨薇. 基于GC-MS和网络药理学探讨当归-肉桂药对的抗炎作用机制[J]. 湖北农业科学, 2022, 61(18): 113-119.
[6]	董雪静, 朱华, 王颖. 基于网络药理学和免疫浸润的大蒜素对肝细胞癌作用机制分析[J]. 湖北农业科学, 2022, 61(10): 83-89.
[7]	许成梅, 陈丹, 徐冬月, 赵庆宇婧, 林玉萍, 赵声兰. 基于网络药理学的核桃防治老年痴呆功能成分及其作用靶点研究[J]. 湖北农业科学, 2021, 60(24): 151-159.
[8]	韩倩, 陈勇, 王竟静, 魏江存, 黄周艳, 陈良妮. 网络药理学方法研究三七治疗关节炎的作用机制[J]. 湖北农业科学, 2021, 60(2): 109-115.
[9]	苏丹, 唐飞, 张善明, 陈玉琼, 倪德江. 不同嫩度青砖茶改善小鼠胃肠道功能研究[J]. 湖北农业科学, 2021, 60(14): 103-108.
[10]	郝爱平, 吴启康, 国会艳, 薛巨坤, 魏继承, 任如意. 基于网络药理学的天南星抗癌机制研究[J]. 湖北农业科学, 2020, 59(23): 110-115.
[11]	唐飞, 张岳峰, 曲凤凤, 陈玉琼, 余志, 倪德江. 不同渥堆程度青砖茶改善小鼠胃肠道功能的研究[J]. 湖北农业科学, 2019, 58(12): 124-129.