湖北农业科学 ›› 2022, Vol. 61 ›› Issue (14): 113-118.doi: 10.14088/j.cnki.issn0439-8114.2022.14.020

• 贮藏·加工 • 上一篇    下一篇

响应面法优化超声辅助提取灵芝黄酮工艺研究

陈芳1, 温艳1, 姜小帆2   

  1. 1.陕西省食品药品检验研究院,西安 710000;
    2.陕西省人民医院,西安 710068
  • 收稿日期:2022-04-24 出版日期:2022-07-25 发布日期:2022-08-25
  • 通讯作者: 姜小帆(1979-),女,陕西西安人,副主任医师,博士,主要从事中药新药临床研究,(电话)18991396015(电子信箱)jiangxf7906@163.com。
  • 作者简介:陈芳(1981-),女,陕西西安人,主管药师,硕士,主要从事中药学及药理学研究,(电话)18629351130(电子信箱)chenfang812021@163.com。
  • 基金资助:
    陕西省重点研发计划项目(2020NY-074); 陕西省中医药管理局科研项目(2021-ZZ-JC011)

Response surface methodology to optimize the ultrasonic-assisted extraction process of Ganoderma lucidum flavonoids

CHEN Fang1, WEN Yan1, JIANG Xiao-fan2   

  1. 1. Shaanxi Institute for Food and Drug Control, Xi'an 710000, China;
    2. Shaanxi Provincial People's Hospital, Xi'an 710068, China
  • Received:2022-04-24 Online:2022-07-25 Published:2022-08-25

摘要: 试验采用响应面法优化超声波辅助提取灵芝(Ganoderma lucidum)中黄酮的工艺。考察乙醇浓度、浸提时间、料液比、超声功率4因素对灵芝中黄酮提取得率的影响,以黄酮得率为响应值,利用Box-Behnken 中心组合设计建立数学模型,进行响应面分析。结果表明,灵芝黄酮的最优提取工艺参数为乙醇浓度86%、料液比1:20 (g/mL)、提取功率185 W、提取时间30 min。在此条件下对建立的数学模型进行验证试验,灵芝黄酮的实际提取得率为7.55%,其与预测值7.62%误差小于3%,因此证实了应用Design-Expert 8.0.6软件得到的黄酮提取工艺参数真实可靠,具有实际应用价值。

关键词: 灵芝, 黄酮, 响应面法, 超声辅助提取

Abstract: The experiment used response surface methodology to optimize the ultrasonic-assisted extraction of flavonoids from Ganoderma lucidum. The effects of four factors, including ethanol concentration, extraction time, solid-liquid ratio, and ultrasonic power, on the extraction yield of flavonoids in Ganoderma lucidum were investigated. Using flavonoids rate as the response value, the Box-Behnken center design developed mathematical model for response surface analysis. The results showed that the optimal extraction process parameters of Ganoderma lucidum flavonoids were ethanol concentration of 86%, solid-liquid ratio of 1:20 (g/mL), extraction power of 185 W, and extraction time of 30 min. The mathematical model established under these conditions was verified by the test. The actual extraction yield of Ganoderma lucidum flavonoids was 7.55%. The error with the predicted value of 7.62% was less than 3%, thus confirming that the flavonoids extraction process parameters obtained with Design-Expert 8.0.6 software were real and reliable, and had practical application value.

Key words: Ganoderma lucidum, flavonoids, response surface methodology, ultrasonic-assisted extraction

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