中国主要油茶产区山茶炭疽菌群体遗传结构分析

doi:10.14088/j.cnki.issn0439-8114.2024.01.038

湖北农业科学 ›› 2024, Vol. 63 ›› Issue (1): 212-217.doi: 10.14088/j.cnki.issn0439-8114.2024.01.038

中国主要油茶产区山茶炭疽菌群体遗传结构分析

王义勋¹, 徐小文¹, 郑露², 黄俊斌², 谢先斌³

1.湖北省林业科学研究院,武汉 430075;
2.华中农业大学植物科技学院,武汉 430070;
3.郧西县林业局,湖北十堰 442600

收稿日期:2022-07-23 出版日期:2024-01-25 发布日期:2024-02-05
作者简介:王义勋（1978-）,男,山东日照人,博士,主要从事林业有害生物防治研究,（电话）027-86952170（电子信箱）yixun2002@163.com。
基金资助:
湖北省重点研发计划资助项目（2020BBA042）

Population genetic structure analysis of camellia anthracis fungus in the main oil tea producing areas of China

WANG Yi-xun¹, XU Xiao-wen¹, ZHENG Lu², HUANG Jun-bin², XIE Xian-bin³

1. Hubei Academy of Forestry, Wuhan 430075,China;
2. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070,China;
3. Yunxi County Forestry Bureau, Shiyan 442600,Hubei,China

Received:2022-07-23 Online:2024-01-25 Published:2024-02-05

摘要/Abstract

摘要： 以中国主要油茶产区的油茶（Camellia oleifera Abel.）炭疽病优势种山茶炭疽菌（Colletotrichum camelliae）的16个地理种群168个菌株为供试材料,应用ISSR分子标记技术进行遗传多样性和种群遗传结构分析。16个地理种群的多态位点百分比（PPB）为98.99%,Nei’s基因多样性指数（He）为0.28,Shannon信息多样性指数（Is）为0.43,遗传相似度（I）平均为0.834,遗传距离（D）平均为0.183,表明山茶炭疽菌遗传多样性水平较高且异质性较强,种群间存在一定程度的遗传变异,遗传距离与地理距离之间无相关性。湖北省、浙江省、江西省、湖南省和广西5个省级种群总基因多样度（Ht）为0.274 8,遗传分化系数（Gst）为0.517 4,基因流（Nm）为0.466 4,表明山茶炭疽菌5个省级种群虽然被分化,但是不存在基因流动现象（Nm<1）。

关键词: 山茶（Camellia oleifera Abel.）, 炭疽菌（Colletotrichum camelliae）, ISSR, 遗传多样性, 种群遗传结构, 油茶产区

Abstract: Using 168 strains from 16 geographical populations of camellia anthracis fungus （Colletotrichum camelliae Abel.）, a dominant species of camellia anthracis in the main camellia producing areas of China, as the test materials, ISSR molecular marker technology was used to analyze genetic diversity and population genetic structure. The percentage of polymorphic loci （PPB） for 16 geographical populations was 98.99%, the Nei’s gene diversity index （He） was 0.28, the Shannon information diversity index （Is） was 0.43, the average genetic similarity （I） was 0.834, and the average genetic distance （D） was 0.183. This indicated that the genetic diversity level and heterogeneity of camellia anthracis fungus were high, and there was a certain degree of genetic variation among populations. There was no correlation between genetic distance and geographical distance. The total genetic diversity （Ht）, genetic differentiation coefficient （Gst）, and gene flow （Nm） of the five provincial-level populations in Hubei, Zhejiang, Jiangxi, Hunan, and Guangxi provinces were 0.274 8, 0.517 4, and 0.466 4, respectively. This indicated that although the five provincial-level populations of camellia anthracis fungus were differentiated, there was no gene flow phenomenon （Nm<1）.

Key words: camellia（Camellia oleifera Abel.）, anthracis fungus（Colletotrichum camelliae）, ISSR, genetic diversity, population genetic structure, oil tea producing areas

中图分类号:

S763.1

王义勋, 徐小文, 郑露, 黄俊斌, 谢先斌. 中国主要油茶产区山茶炭疽菌群体遗传结构分析[J]. 湖北农业科学, 2024, 63(1): 212-217.

WANG Yi-xun, XU Xiao-wen, ZHENG Lu, HUANG Jun-bin, XIE Xian-bin. Population genetic structure analysis of camellia anthracis fungus in the main oil tea producing areas of China[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(1): 212-217.

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中国主要油茶产区山茶炭疽菌群体遗传结构分析

Population genetic structure analysis of camellia anthracis fungus in the main oil tea producing areas of China

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