湖北农业科学 ›› 2024, Vol. 63 ›› Issue (4): 30-36.doi: 10.14088/j.cnki.issn0439-8114.2024.04.006

• 资源·环境 • 上一篇    下一篇

一株高效解钾菌的筛选、鉴定及培养条件的优化

张雷1, 邱路凡1, 刘丽红1, 赵雪钰2, 韩春梅3   

  1. 1.东北石油大学地球科学学院,黑龙江 大庆 163319;
    2.湖北工业大学,武汉 430068;
    3.大庆油田第三采油厂工艺研究所,黑龙江 大庆 163000
  • 收稿日期:2023-05-08 出版日期:2024-04-25 发布日期:2024-05-07
  • 作者简介:张雷(1982-),男,黑龙江大庆人,教授,博士,主要从事土壤调查、保护与修复技术研究,(电话)13614590682(电子信箱)zhlei162@126.com。
  • 基金资助:
    国家自然基金项目(41877489); 黑龙江省优秀青年基金项目(YQ2020D003)

Screening, identification and culture conditions optimization of a highly efficient potassium-solubilizing bacteria

ZHANG Lei1, QIU Lu-fan1, LIU Li-hong1, ZHAO Xue-yu2, HAN Chun-mei3   

  1. 1. School of Earth Sciences, Northeast Petroleum University, Daqing 163319,Heilongjiang,China;
    2. Hubei University of Technology, Wuhan 430068,China;
    3. Technology Research Institute of the Third Oil Production Plant of Daqing Oilfield, Daqing 163000,Heilongjiang,China
  • Received:2023-05-08 Online:2024-04-25 Published:2024-05-07

摘要: 以黑土水稻田中解钾菌为研究目标,共分离筛选出5株解钾菌,通过其形态特征以及测定菌株解钾能力筛选出1株高效解钾菌株S1。利用16S rDNA鉴定S1的种属,并通过单因素试验和正交试验优化该菌株的培养条件。结果表明,分离筛选得到的5株解钾菌均能有效分解钾长石,菌株S1解钾能力最强,解钾率为57.03%,发酵液中解钾量为3.852 mg/L,为最优解钾菌。初步鉴定解钾菌S1为伯克霍尔德菌(Burkholderia ambifaria)。经单因素试验和正交试验优化后的培养组分为碳源1.0%甘露醇、氮源1.0%蛋白胨、无机盐0.5%K2HPO4,培养条件为温度30 ℃、时间48 h、培养基装液量(培养瓶250 mL) 80 mL、初始pH 6.5、接种量5.0%。

关键词: 黑土, 解钾菌, 菌种鉴定, 培养条件, 正交试验

Abstract: Five strains of potassium-solubilizing bacteria were isolated and screened from black soil paddy field. One strain (S1)with high efficiency of potassium-solubilizing was screened by its morphological characteristics and potassium-solubilizing ability. The species of S1 was identified by 16S rDNA, and the culture conditions of the strain were optimized by the single factor test and orthogonal test. The results showed that the five strains of potassium-solubilizing bacteria were all able to decompose potassium feldspar effectively. Strain S1 had the highest potassium-solubilizing ability with a potassium-solubilizing rate of 57.03%, and the content of potassium-solubilizing in the fermentation broth was 3.852 mg/L, which was the best potassium-solubilizing bacteria. The potassium-solubilizing strain S1 was identified as Burkholderia ambifaria. The optimized culture conditions were carbon source 1.0% mannitol, nitrogen source 1.0% peptone, inorganic salt 0.5% K2HPO4, culture temperature 30 ℃, culture time 48 h, liquid volume 80 mL/250 mL, initial pH 6.5, and inoculation volume 5.0%.

Key words: lack soil, potassium-solubilizing bacteria, strain identification, culture conditions, orthogonal test

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