湖北农业科学 ›› 2024, Vol. 63 ›› Issue (4): 154-157.doi: 10.14088/j.cnki.issn0439-8114.2024.04.028

• 检测分析 • 上一篇    下一篇

壮药广西鹅掌柴质量标准建立

温海成, 苏永精, 谢澳, 李灿, 韦威   

  1. 广西中医药大学壮医药学院,南宁 530200
  • 收稿日期:2023-04-11 出版日期:2024-04-25 发布日期:2024-05-07
  • 通讯作者: 韦威(1981-),男,广西藤县人,高级实验师,主要从事中药、民族药研究与开发工作,(电话)13100510488(电子信箱)65462479@qq.com。
  • 作者简介:温海成(1979-),女,黑龙江双鸭山人,教授,硕士,主要从事民族药、中药鉴定及品质评价研究,(电话)13877166296(电子信箱)wenhaicheng2015@qq.com。
  • 基金资助:
    广西壮族自治区科学技术厅重点研发计划项目(桂科AB19110027); 广西壮族自治区科学技术厅广西中药药效研究重点实验室项目(20-065-38)

Establishment of quality standards for Zhuang medicine Schefflera kwangsiensis

WEN Hai-cheng, SU Yong-jing, XIE Ao, LI Can, WEI Wei   

  1. School of Zhuang Medicine, Guangxi University of Chinese Medicine,Nanning 530200,China
  • Received:2023-04-11 Online:2024-04-25 Published:2024-05-07

摘要: 采用显微鉴别和薄层色谱鉴别方法对不同产地壮药广西鹅掌柴进行鉴定研究,按照《中华人民共和国药典》2020年版四部通则中规定的方法对不同产地药材进行水分含量、灰分含量和浸出物含量的测定。结果表明,壮药广西鹅掌柴显微鉴别特征性强;薄层色谱鉴别方法以环己烷∶丙酮∶甲酸(8.5∶1.5∶0.4)为展开系统,此展开系统分离度较好,斑点清晰,以15 cm为展距,斑点分离度最佳;以10%硫酸乙醇溶液作为显色剂,紫外灯(365 nm)下检视;拟定壮药广西鹅掌柴水分含量不超过12.00%,总灰分含量不超过6.00%,酸不溶性灰分含量不超过0.50%,水溶性浸出物含量不低于9.50%。其方法操作简单易行、稳定可靠、重复性较好、拟定范围合理,可为壮药广西鹅掌柴质量控制提供理论依据。

关键词: 壮药, 广西鹅掌柴, 质量标准, 薄层色谱

Abstract: Microscopic identification and thin-layer chromatography identification methods were used to identify and study the Zhuang medicine Schefflera kwangsiensis from different regions. The moisture content, ash content, and extract content of the medicinal materials from different regions were determined according to the methods specified in the 2020 edition of the "Pharmacopoeia of the People’s Republic of China". The results showed that the microscopic identification characteristics of the Zhuang medicine Schefflera kwangsiensis were strong; the thin-layer chromatography identification method used cyclohexane∶acetone∶formic acid of 8.5∶1.5∶0.4 as the unfolding system, which had good separation and clear spots. The best spot separation was achieved with a spreading distance of 15 cm;using a 10% sulfuric acid ethanol solution as the chromogenic agent, the examination was conducted under a UV lamp (365 nm); the moisture content of Zhuang medicine Schefflera kwangsiensis shall not exceed 12.00%, the total ash content should not exceed 6.00%, the acid insoluble ash content should not exceed 0.50%, and the water-soluble extract content should not be less than 9.50%. The method was easy to operate, stable and reliable, with good reproducibility and reasonable scope, which could provide a theoretical basis for the quality control of Zhuang medicine Schefflera kwangsiensis.

Key words: Zhuang medicine, Schefflera kwangsiensis, quality standards, thin-layer chromatography

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