基于SSR荧光标记的121份野生枸杞种质遗传结构解析

doi:10.14088/j.cnki.issn0439-8114.2024.05.036

摘要/Abstract

摘要： 以采集的121份野生枸杞（Lycium barbarum L.）种质为材料,用9对SSR引物进行扩增,采用GeneALEX、Power Marker软件进行多态性分析,利用NTSYS、STRUC-TURE软件进行遗传多样性分析、聚类分析和主成分分析。结果表明,9对SSR引物共检测到108个等位基因,等位基因数（Na）、有效等位基因数（Ne）、观测杂合度（Ho）、期望杂合度（He）、多态信息含量（PIC）、Shannon’s信息指数分别为12个、4.620个、0.678、0.651、0.629、1.568;聚类结果显示121份野生枸杞种质可划分为5个亚群,群体遗传结构表明当K=5时,121个种质划分为5个亚群,来自宁夏的NXGY-02、NXGY-03 2个种质与来自内蒙古的6个种质之间的遗传距离最远,亲缘关系较远,来自青海省、新疆、甘肃省及内蒙古的其余野生枸杞种质之间存在广泛的基因交流现象;宁夏、内蒙古的野生枸杞种质具有独立起源进化的可能,而新疆、甘肃省、青海省的种质与宁夏种质亲缘关系较近,很可能属于同一起源。

关键词: 野生枸杞（Lycium barbarum L.）, 种质, SSR荧光标记, 遗传结构

Abstract: 121 wild goji berry（Lycium barbarum L.） germplasms were collected as materials, 9 pairs of SSR primers were used of amplification, and polymorphism was analyzed using GeneALEX and Power Marker software. Genetic diversity analysis, cluster analysis, and principal component analysis were performed using NTSYS and STRUC-TURE software.The results showed that a total of 108 alleles were detected using 9 pairs of SSR primers. The number of alleles （Na）, effective alleles （Ne）, observed heterozygosity （Ho）, expected heterozygosity （He）, polymorphic information content （PIC）, and Shannon’s information index were 12, 4.620, 0.678, 0.651, 0.629, and 1.568, respectively;the clustering results showed that 121 wild goji berry germplasms could be divided into 5 subgroups. The genetic structure of the population showed that when K=5, 121 germplasms could be divided into 5 subgroups. The genetic distance between NXGY-02 and NXGY-03 germplasms from Ningxia and 6 germplasms from Inner Mongolia was the farthest, and the genetic relationship was relatively distant,there was extensive gene exchange among other wild goji berry germplasms from Qinghai Province, Xinjiang, Gansu Province, and Inner Mongolia;the wild goji berry germplasms in Ningxia and Inner Mongolia had the possibility of independent origin and evolution, while the germplasms in Xinjiang, Gansu, and Qinghai provinces were closely related to the germplasms in Ningxia and were likely to belong to the same origin.

Key words: wild goji berrie （Lycium barbarum L.）, germplasms, SSR fluorescence markers, genetic structure

中图分类号:

S326

戴国礼, 刘娜, 秦垦, 张波, 尹跃, 米佳, 何昕孺. 基于SSR荧光标记的121份野生枸杞种质遗传结构解析[J]. 湖北农业科学, 2024, 63(5): 207-214.

DAI Guo-li, LIU Na, QIN Ken, ZHANG Bo, YIN Yue, MI Ja, HE Xin-ru. Genetic structure analysis of 121 wild goji berry germplasms based on SSR fluorescence markers[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(5): 207-214.

参考文献

[1] 张欣,王鑫,刘辉鹏,等.宁夏枸杞雄性不育品系和可育品系工厂化快繁体系的建立[J/OL].分子植物育种,2022.https://kns.cnki.net/kcms2/article/abstract?v=M7N75Hb03FX6fvGCfBn4Uer5v 3RLUDId7BEkT3bJ5OVaXfyx_fjowXkGJvHsBWfwmNwA16JGL8z AVy4BIa3fXka1GzZ4nlenlwu5qMPjgT9r_BDGQPqHcGvUDFrzNtmqJ7yvieQ3lWI=&uniplatform=NZKPT&language=CHS.
[2] 肖佳,高昊,周正群,等.枸杞属中枸杞红素类成分研究进展[J].科学通报,2017,62(16):1691-1698.
[3] 张兴,冯嘉馨,陈佳,等.雄性不育枸杞花药比较转录组分析[J].基因组学与应用生物学,2022,41(6):1274-1285.
[4] 陈艳瑞,尹林克.人工防风固沙林演替中群落组成和优势种群生态位变化特[J].植物生态学报,2008(5):1126-1133.
[5] 袁惠君,刘轲,王春梅,等.两个宁夏枸杞品种的耐渗透胁迫和耐盐特征比较[J].草业科学,2016,33(4):681-690.
[6] FAKHFAKH J,ATHMOUNI K,MALLEK-FAKHFAKH H, et al.Polysaccharide from Lycium barbarum: Structural features, in vitro antioxidant activities and protective effect against oxidative damage in human erythrocytes[J].Chemistry and biodiversity,2020,17(12):e2000614.
[7] 裴红霞,高晶霞,王学梅.220个辣椒种质形态学性状的遗传多样性分析[J].分子植物育种,2022,20(4):1331-1347.
[8] 尹莉岚,姚方杰,石晨阳,等.基于SSR标记的野生黑木耳种质资源遗传多样性研究[J].食用菌学报, 2022, 29(4):1-9.
[9] HAMADA H, PETRINO M G, KAKUNAGA T.A novel repeated element with Z-DNA-forming potential is widely found in evolutionarily diverse eukaryotic genomes[J].Proceedings of the national academy of sciences,1982,79(21):6465-6469.
[10] ZIETKIEWICZ E, RAFALSKI A, LABUDA D.Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification[J].Genomics,1994,20(2):176-183.
[11] BOTSTEIN D, WHITE R L, SKOLNICK M, et al.Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J].American journal of human genetics,1980, 32(3):314.
[12] VOS P, HOGERS R, BLEEKER M, et al.AFLP: A new technique for DNA fingerprinting[J]. Nucleic acids research, 1995, 23(21): 4407-4414.
[13] 蔡海滨,韩光煜,涂敏,等.基于SSR荧光标记的江苏省杂草稻群体遗传多样性分析[J].分子植物育种,2022,20(21):7104-7115.
[14] GUR-ARIE R, COHEN C J, EITAN Y,et al.Simple sequence repeats in Escherichia coli:Abundance, distribution, composition, and polymorphism[J].Genome research,2000,10(1):62-71.
[15] MCCOUCH S R,CHEN X,PANAUD O, et al.Microsatellite marker development, mapping and applications in rice genetics and breeding[J].Plant molecular biology,1997,35:89-99.
[16] 鲍红春,李小雷,王建平,等.枸杞遗传多样性的ISSR分析[J].华北农学报,2014,29(1):89-92.
[17] FALAHATI-ANBARAN M,HABASHI A,ESFAHANY M, et al.Population genetic structure based on SSR markers in alfalfa (Medicago sativa L.) from various regions contiguous to the centres of origin of the species[J]. Journal of genetics,2007,86(1):59.
[18] 梁燕,韩传明,周继磊,等.山东核桃良种SSR指纹图谱及分子身份证的构建——基于毛细管电泳分析[J].中国农学通报,2022,38(15):113-121.
[19] 石纹豪,黄振霖,杨水英,等.重庆马铃薯致病疫霉(Phytophthora infestans)的SSR基因型分析[J].西南大学学报(自然科学版),2014, 36(12):36-41.
[20] CHIOU C Y, SHIH H C, TSAI C C, et al.The genetic relationships of Indian jujube (Ziziphus mauritiana Lam.) cultivars using SSR markers[J].Heliyon,2020,6(10):e05078.
[21] 石志刚,安巍,焦恩宁,等.基于nrDNA ITS序列的18个宁夏枸杞资源的遗传多样性[J].安徽农业科学,2008,(24):10379-10380.
[22] 李彦龙,樊云芳,戴国礼,等.枸杞种质遗传多样性的AFLP分析[J].中草药,2011,42(4):770-773.
[23] 安巍,王亚军,尹跃,等.枸杞种质资源的SRAP分析[J].浙江农业学报,2013,25(6):1234-1237.
[24] 尹跃,安巍,赵建华,等.枸杞品种SSR荧光指纹图谱构建及遗传关系分析[J].西北林学院学报,2017,32(1):137-141.
[25] 仲小茹,柯叮,黄献峰,等.基于SSR标记的江西省枫香古树遗传多样性评价[J].植物遗传资源学报,2023,24(2):523-531.
[26] 聂兴华,李伊然, 田寿乐,等.中国板栗品种(系)DNA指纹图谱构建及其遗传多样性分析[J].园艺学报,2022,49(11):2313-2324.
[27] 张雨朋,丁冬会,李蒙鑫, 等.河南地方绿豆种质资源遗传多样性的SSR分析[J/OL].分子植物育种,2022.https://kns.cnki.net/kcms2/article/abstract?v=M7N75Hb03FWQfkOzfXDl0o_mILbmC 8zM25JjcGkBBB_3poIuoFBJVQ68elZjwlH4AZvYpMqM1I-oNfug 9QV9VfNKxf3mX9XeIgJUBgTxF4zSp9OrGLHZS-Hm-1iJGoIiwG Nd-9U2QpE=&uniplatform=NZKPT&language=CHS.
[28] 张旻桓,姚奕平,黄宇,等.基于SSR标记的江南牡丹品种群遗传多样性及亲缘关系[J].中南林业科技大学学报,2023, 43(1):164-172.
[29] 胡永超,马洁,唐建宁,等.不同树龄枸杞古树的遗传多样性研究[J].植物遗传资源学报,2022,23(3):755-67.
[30] 秦英之,车佳航,尹跃,等.基于全长转录组信息的枸杞SSR标记开发[J].植物遗传资源学报,2022,23(6):1816-1827.
[31] 王红梅,陈玉梁,马彦军,等.西北荒漠区野生黑果枸杞种质资源遗传多样性分析[J].中国野生植物资源, 2020,39(11):27-33,38.
[32] 黄兴发,尹跃,赵建华,等.黑果枸杞基因组SSR标记开发及遗传多样性分析[J].西北农林科技大学学报(自然科学版),2021,49(1):126-135.
[33] 卢俊浩,张婷,张鹏,等.基于SSR标记的花菜类作物遗传多样性及群体结构分析[J].西南农业学报,2022,35(8):1887-1894.