关键词: 美味猕猴桃（Actinidia deliciosa）, AcMYB, 鉴定, 水淹胁迫, 响应

Abstract: Based on the second-generation transcriptome sequencing data of Actinidia deliciosa, AcMYB family genes were identified and analyzed using various bioinformatics methods. The results showed that a total of 64 AcMYB family members with complete ORF sequences were obtained from the transcriptome data of Actinidia deliciosa, which were divided into 4 subgroups. Among them, subgroup I had 34 members, subgroup II had 18 members, subgroup III had 10 members, and subgroup IV had 2 members. Expression analysis before and after waterlogging treatment showed that the expression levels of AcMYB members such as AcMYB214187 and AcMYB25788 were significantly reduced in the roots of Actinidia deliciosa, responding quickly to waterlogging stress, while there was no significant difference in expression levels in the leaves. The expression levels in the roots of AcMYB members such as AcMYB19450 and AcMYB30021 were not significantly different, but the expression levels in the leaves were significantly reduced. This might be due to the fact that the roots, upon receiving hypoxic stress signals, transmitted them to the aboveground parts and exerted their effects, regulating the response of Actinidia deliciosa to hypoxic stress at the transcriptional level. The research results provided a basis for further research on MYB transcription factors in Actinidia deliciosa, promoting stress resistance breeding, and improving yield.

Key words: Actinidia deliciosa, AcMYB, identification, flooding stress, response