猕猴桃‘脐红'与‘徐香'越冬期转录组的比较分析

doi:10.14088/j.cnki.issn0439-8114.2023.05.029

湖北农业科学 ›› 2023, Vol. 62 ›› Issue (5): 165-171.doi: 10.14088/j.cnki.issn0439-8114.2023.05.029

猕猴桃‘脐红'与‘徐香'越冬期转录组的比较分析

毛可欣^1,2, 王海荣¹, 安淼¹, 吕巍², 李健¹, 李国田¹

1.山东省果树研究所,山东泰安 271000;
2.山东农业大学生命科学学院,山东泰安 271000

收稿日期:2021-12-06 出版日期:2023-05-25 发布日期:2023-06-12
通讯作者: 李健（1991-）,男,助理研究员,博士,主要从事猕猴桃育种研究,（电话）15600804619（电子信箱）lijian097597@163.com;李国田（1980-）,男,副研究员,主要从事猕猴桃栽培与育种研究,（电话）13668680270（电子信箱）ligt2008@163.com。
作者简介:毛可欣（1999-）,女,山东临沂人,在读硕士研究生,研究方向为猕猴桃育种,（电话）15646703765（电子信箱）mao_kx@foxmail.com。
基金资助:
中央引导地方科技发展专项（YDZX20193700004330）; 山东省农业科学院科技创新工程项目（CXGC2021A31）; 山东省农业科学院科技创新工程-人才项目（CXGC2021B34）

Comparative analysis of the transcriptome of kiwifruit ‘Qihong' and ‘Xuxiang' during the overwintering period

MAO Ke-xin^1,2, WANG Hai-rong¹, AN Miao¹, LV Wei², LI Jian¹, LI Guo-tian¹

1. Shandong Institute of Pomology, Tai'an 271000,Shandong,China;
2. College of Life Sciences,Shandong Agricultural University, Tai'an 271000,Shandong,China

Received:2021-12-06 Online:2023-05-25 Published:2023-06-12

摘要/Abstract

摘要： 以不同抗寒能力的中华猕猴桃（Actinidia chinensis）‘脐红'和美味猕猴桃（Actinidia deliciosa）‘徐香'为材料,在越冬期取样并分析生理指标,发现‘脐红'低温耐受性高于‘徐香'。对2个品种的转录组进行分析,GO聚类中细胞成分组织或生物生成、细胞器部分等差异较大;KEGG聚类中膜运输、翻译、转录、复制和修复等差异较大。对2个品种进行富集分析,淀粉和蔗糖代谢、植物激素信号转导、苯丙类化合物的生物合成和黄酮类化合物的生物合成通路存在差异表达,解析了2个品种在越冬期转录水平的变化。

关键词: 中华猕猴桃（Actinidia chinensis）, 美味猕猴桃（Actinidia deliciosa）, 低温胁迫, 越冬期, 转录组

Abstract: Chinese kiwifruit （Actinidia chinensis） ‘Qihong' and delicious kiwifruit （Actinidia deliciosa） ‘Xuxiang' with different cold tolerance were used as materials.Samples were taken during the overwintering period and analyzed for physiological parameters, and it was found that ‘Qihong' was more tolerant to low temperatures than ‘Xuxiang'. The transcriptomes of the two varieties were analyzed and the GO clusters showed greater differences in cellular components organization or biogenesis, organelle fraction, etc;KEGG clusters differed more in membrane transport, translation, transcription, replication and repair. The enrichment analysis of the 2 varieties showed differential expression of starch and sucrose metabolism, phytohormone signaling, biosynthesis of phenylpropanoids and biosynthesis of flavonoids pathways. The changes in transcript levels of the two varieties during the overwintering period were analyzed.

Key words: chinese kiwifruit （Actinidia chinensis）, delicious kiwifruit （Actinidia deliciosa）, low temperature stress, overwintering period, transcriptome

中图分类号:

S663

毛可欣, 王海荣, 安淼, 吕巍, 李健, 李国田. 猕猴桃‘脐红'与‘徐香'越冬期转录组的比较分析[J]. 湖北农业科学, 2023, 62(5): 165-171.

MAO Ke-xin, WANG Hai-rong, AN Miao, LV Wei, LI Jian, LI Guo-tian. Comparative analysis of the transcriptome of kiwifruit ‘Qihong' and ‘Xuxiang' during the overwintering period[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(5): 165-171.

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猕猴桃‘脐红'与‘徐香'越冬期转录组的比较分析

Comparative analysis of the transcriptome of kiwifruit ‘Qihong' and ‘Xuxiang' during the overwintering period

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