类黄酮3′-羟化酶及其在大麦中的研究进展

doi:10.14088/j.cnki.issn0439-8114.2025.03.005

摘要/Abstract

摘要： 类黄酮3′-羟化酶（F3′H）是植物黄酮类化合物中的花青素和原花青素合成代谢中的关键酶,在植物的花色和果色修饰、抗逆、抗病虫等方面有重要作用。大麦（Hordeum vulgare L.）富含黄酮物质,具有保健功效,对大麦F3′H基因进行相关研究对培育富含黄酮物质的药食两用大麦品种有重要意义。介绍了F3′H的发现历程以及在花青素和原花青素合成途径中的作用;在基因层面的相关研究中,已有多种植物的F3′H基因被克隆出来,部分研究对该基因的形成以及进化历程进行了分析,部分研究揭示了与F3′H基因相关的转录因子以及其在不同部位、不同外界环境条件胁迫下的表达情况;在部分植物中F3′H基因的表达可提高植物对逆境胁迫以及部分病虫害的抵抗能力;大麦中黄酮合成途径中的相关结构基因基本都已被克隆出来,F3′H基因是其中较晚被克隆出来的,该基因已有多个变体从一些大麦品种中被克隆出来并进行了相关研究,这些研究对培育富含黄酮的大麦品种具有重要意义。

关键词: 类黄酮3′-羟化酶（F3′H）, 黄酮, 代谢, 大麦（Hordeum vulgare L.）

Abstract: Flavonoid 3′-hydroxylase (F3′H) is a key enzyme in the biosynthetic pathway of anthocyanins and proanthocyanidins in plant flavonoids, playing a crucial role in modifying flower and fruit colors, as well as enhancing stress resistance and pest resistance in plants. Barley （Hordeum vulgare L.） is rich in flavonoids and possesses health-promoting properties, and research on the F3′H gene in barley is significant for the development of medicinal and edible barley varieties rich in flavonoids. This review briefly introduced the discovery history of F3′H and its role in the biosynthetic pathway of anthocyanins and proanthocyanidins. At the genetic level, the F3′H gene had been cloned from various plants. Some studies have analyzed the formation and evolutionary history of this gene, while others have revealed the transcription factors associated with F3′H and its expression patterns in different tissues and under various environmental stress conditions. In some plants, the expression of the F3′H gene could enhance resistance of plants to stress and some pests and diseases. In barley, most of the structural genes involved in the flavonoid biosynthetic pathway have been cloned, with F3′H being one of the later genes to be cloned. Currently, multiple variants of this gene have been cloned and studied from several barley varieties, and these studies are of great significance for the future breeding of barley varieties rich in flavonoids.

Key words: flavonoid 3′-hydroxylase(F3′H), flavone, metabolism, barley（Hordeum vulgare L.）

中图分类号:

赵炜, 景兴怀, 杨涛, 陈佳, 普晓英, 杨晓梦, 李娥贤, 李霞, 杨丽娥, 曾亚文. 类黄酮3′-羟化酶及其在大麦中的研究进展[J]. 湖北农业科学, 2025, 64(3): 29-35.

ZHAO Wei, JING Xing-huai, YANG Tao, CHEN Jia, PU Xiao-ying, YANG Xiao-meng, LI E-xian, LI Xia, YANG Li-e, ZENG Ya-wen. Research progress of flavonoid 3′-hydroxylase and its applications in barley[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(3): 29-35.

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