栎黄枯叶蛾诱导沙棘挥发性物质释放节律

doi:10.14088/j.cnki.issn0439-8114.2025.09.014

湖北农业科学 ›› 2025, Vol. 64 ›› Issue (9): 84-91.doi: 10.14088/j.cnki.issn0439-8114.2025.09.014

栎黄枯叶蛾诱导沙棘挥发性物质释放节律

唐硕, 许波喜, 冯江帅, 安科旭, 刘永华

榆林学院现代农学院,陕西榆林 719000

收稿日期:2025-04-23 出版日期:2025-09-25 发布日期:2025-10-28
通讯作者: 刘永华(1978-),男,山西阳泉人,教授,博士,主要从事作物有害生物控制研究,(电子信箱)liuyonghuaa@126.com。
作者简介:唐硕(1997-),女,黑龙江鸡西人,在读硕士研究生,研究方向为作物有害生物控制,(电子信箱)tangssss@qq.com。
基金资助:
国家自然科学基金项目(32260398); 榆林学院研究生创新基金一般项目(2024YLYCX16)

The release rhythm of Hippophae rhamnoides volatile compounds induced by Trabala vishnou gigantina Yang

TANG Shuo, XU Bo-xi, FENG Jiang-shuai, AN Ke-xu, LIU Yong-hua

College of Advanced Agricultural Sciences, Yulin University, Yulin 719000, Shaanxi, China

Received:2025-04-23 Published:2025-09-25 Online:2025-10-28

摘要/Abstract

摘要： 系统解析受害沙棘(Hippophae rhamnoides)与健康沙棘之间挥发物组成的差异,研究栎黄枯叶蛾(Trabala vishnou gigantina Yang)诱导沙棘挥发物释放的日节律变化。通过动态顶空收集,结合气相色谱-质谱联用技术(GC-MS)分析,在虫害沙棘中检测出33种挥发物,相比健康沙棘多3种新的挥发物,分别为马兜铃烯、葎草烯、2-甲基丁酸己酯。从释放节律来看,受害沙棘挥发物释放高峰集中于6:00—9:00,而健康沙棘挥发物释放高峰集中于24:00—3:00,9:00—12:00虫害诱导挥发物和健康沙棘挥发物均未出现释放最高峰挥发物。筛选出昼夜释放差异显著的马兜铃烯、2-甲基丁酸己酯、葎草烯、乙酸叶醇酯、2-乙基己醇、长叶烯6种虫害诱导挥发物的释放高峰均出现在夜间。各挥发物释放具有不同的昼夜节律性,挥发物的释放量既受到植株受害的影响,又受到昼夜节律的影响,普遍表现为夜间释放量高,白天释放量低。

关键词: 沙棘(Hippophae rhamnoides), 栎黄枯叶蛾(Trabala vishnou gigantina Yang), 虫害诱导挥发物, 昼夜释放节律

Abstract: The differences in volatile organic compound (VOC) profiles between herbivore-infested and healthy sea buckthorn (Hippophae rhamnoides) plants were systematically analyzed, with particular emphasis placed on the circadian rhythm of VOC release induced by Trabala vishnou gigantina Yang. Dynamic headspace collection combined with gas chromatography-mass spectrometry (GC-MS) was used for analysis. Thirty-three volatiles were detected in infested plants, among which three compounds:Aristolochene, humulene, and hexyl 2-methylbutyrate were newly identified compared to those in healthy plants. Regarding release rhythm, peak volatile release was observed in damaged plants between 6:00 and 9:00, while in healthy plants, release peaks were recorded between 24:00 and 3:00. No release peak was observed in either group between 9:00 and 12:00. Six herbivore-induced volatiles showing significant diurnal variation:Aristolochene, hexyl 2-methylbutyrate, humulene, cis-3-hexenyl acetate, 2-ethylhexanol, and longifolene were all found to peak during the night. Distinct diurnal release patterns were exhibited by each volatile, which were influenced by both plant damage status and circadian rhythm. Generally, higher release levels were observed at night, and lower levels were detected during the day.

Key words: Hippophae rhamnoides, Trabala vishnou gigantina Yang, herbivore-induced plant volatiles(HIPVs), circadian release rhythm

中图分类号:

唐硕, 许波喜, 冯江帅, 安科旭, 刘永华. 栎黄枯叶蛾诱导沙棘挥发性物质释放节律[J]. 湖北农业科学, 2025, 64(9): 84-91.

TANG Shuo, XU Bo-xi, FENG Jiang-shuai, AN Ke-xu, LIU Yong-hua. The release rhythm of Hippophae rhamnoides volatile compounds induced by Trabala vishnou gigantina Yang[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(9): 84-91.

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栎黄枯叶蛾诱导沙棘挥发性物质释放节律

The release rhythm of Hippophae rhamnoides volatile compounds induced by Trabala vishnou gigantina Yang

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