湖北农业科学 ›› 2018, Vol. 57 ›› Issue (20): 79-84.doi: 10.14088/j.cnki.issn0439-8114.2018.20.017

• 植物保护 • 上一篇    下一篇

花椒窄吉丁后肠、粪便挥发物的提取与鉴定

巩雪芳, 谢寿安, 车显荣, 刘绥鹏, 吕淑杰   

  1. 西北农林科技大学林学院,陕西 杨凌 712100
  • 收稿日期:2018-03-16 发布日期:2018-10-25
  • 通讯作者: 谢寿安,教授,(电子信箱)shouanxie@163.com。
  • 作者简介:巩雪芳(1993-),女,甘肃天水人,在读硕士研究生,研究方向为森林昆虫学,(电话)15029989035(电子信箱)244315334@qq.com
  • 基金资助:
    国家公益性行业(林业)项目(201504324)

Extraction and Identification of Volatiles Substances in Hindgut and Feces of Agrilus zanthoxylumi

GONG Xue-fang, XIE Shou-an, CHE Xian-rong, LIU Sui-peng, LV Shu-jie   

  1. College of Forestry,Northwest Agriculture & Forestry University,Yangling 712100,Shaanxi,China
  • Received:2018-03-16 Published:2018-10-25

摘要: 采用正己烷浸提法提取花椒窄吉丁(Agrilus zanthoxylumi)后肠、粪便挥发物,应用GC-MS技术分析其化学组分和相对含量。结果表明,花椒窄吉丁成虫后肠挥发物种类和相对含量雌雄差异不大,主要为醛类和有机酸化合物,顺-9-十六烯醛在雌雄虫后肠挥发物中相对含量最高。雌虫粪便挥发物以烃类和醇类化合物为主,分别以正四十烷(21.16%)和叶绿醇(8.53%)相对含量为最高;雄虫以有机酸和烃类为主,分别以油酸(24.74%)和二十一烷(6.07%)相对含量为最高。雌、雄虫粪便挥发物种类和相对含量差异很大。

关键词: 花椒窄吉丁(Agrilus zanthoxylumi), 正己烷, GC-MS, 后肠, 粪便

Abstract: Studying the specific component composition and relative content of the volatile components in the hindgut and feces of Agrilus zanthoxylumi,the volatile components were extracted by n-hexane and which were analyzed by GC-MS. The results showed that the main components of the volatile substances in the hindgut of females and males were aldehydes and organic acids. The relative content of cis-9-hexadecenal in the hindgut volatiles from females and males was the highest. The differences in species and relative content of feces volatile from females and males were great,the main components of the femals feces volatile were hydrocarbons and alcohols,tetracontane(21.16%) and phytol(8.53%) were the highest,while hydrocarbons and organic acids were the main components in males,oleic acid(24.74%) and heneicosane(6.07%) were the highest. The species and relative contents of volatile matter in feces of female and male insects were very different.

Key words: Agrilus zanthoxylumi, n-hexane, GC-MS, hindgut, feces

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