PGAM5基因核心启动子区域鉴定与转录调控分析

doi:10.14088/j.cnki.issn0439-8114.2025.10.025

摘要/Abstract

摘要： 为分析猪流行性腹泻病毒（Porcine epidemic diarrhea virus,PEDV）感染后对细胞内磷酸甘油酸变位酶5（Phosphoglyceratemutase5,PGAM5）表达的调控作用,完善PEDV病毒的作用机制,通过qRT-PCR对PEDV感染LLC-PK1细胞后PGAM5表达量进行分析,并采用步移法对启动子核心区域进行筛选,通过JASPAR数据库筛选与PGAM5基因核心启动子结合的转录因子并采用qRT-PCR对筛选的基因进行初步验证。结果表明,PEDV感染LLC-PK1细胞后,PGAM5基因mRNA表达量上调,筛选得到PGAM5启动子核心区域位于-60 bp到-1 bp之间。通过qRT-PCR初步验证转录因子TFAP2A、TCFL5、ZNF354C、ARNT对PGAM5启动子具有转录调控作用。

关键词: PEDV, PGAM5, 核心启动子, 转录因子

Abstract: To analyze the regulation of intracellular PGAM5 expression after PEDV infection and to refine the mechanism of action of PEDV virus. In this experiment, the expression of PGAM5 after PEDV infection of LLC-PK1 cells were analyzed by qRT-PCR, and screened the core region of the promoter by step-wise method, and screened the transcription factors that bind to the core promoter of the PGAM5 gene by the JASPAR database and preliminarily verified the screened genes by qRT-PCR. The results showed that PGAM5 gene mRNA expression was up-regulated after PEDV infection of LLC-PK1 cells. The core region of PGAM5 promoter was screened to be located between -60 bp and -1 bp. The transcription factors TFAP2A, TCFL5, ZNF354C and ARNT were preliminarily verified to have transcriptional regulation of the PGAM5 promoter by qRT-PCR.

Key words: PEDV, PGAM5, core promoter, transcription factor

中图分类号:

S852.65

李泽亚. PGAM5基因核心启动子区域鉴定与转录调控分析[J]. 湖北农业科学, 2025, 64(10): 165-170.

LI Ze-ya. Identification of PGAM5 gene core promoter and analysis of transcriptional regulation[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(10): 165-170.

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