关键词: 大叶黄精（Polygonatum kingianum var. grandifolium）, 黄精（Polygonatum sibiricum Red）, 有机酸, bHLH转录因子, 生物信息学, 家族, 鉴定

Abstract: The transcriptomes of Polygonatum kingianum var. grandifolium and Polygonatum sibiricum Red were sequenced using a sequencing platform. Bioinformatics methods were used to identify the bHLH transcription factors family related to organic acid metabolism, and the predicted proteins were analyzed for their physicochemical properties, structural domains, and phylogenetic relationships. The results showed that based on the transcriptome data of Polygonatum kingianum var. grandifolium and Polygonatum sibiricum Red, a total of 46 682 transcription factors were obtained, including 110 bHLH transcription factors; there were 978 transcription factors with significant differences in the total transcription data, including 53 bHLH transcription factors;based on metabolomic prediction data, 31 transcription factors related to organic acid were identified, including 22 bHLH transcription factors related to organic acid. Except for Cluster-43865.11, Motif 1 was distributed in all other sequences, and the vast majority of sequences contained Motif 4; all 22 members of the bHLH transcription factors family exhibited varying degrees of conserved motif variation, with each bHLH containing 1-14 Motifs and an average of 7.81 Motifs.

Key words: Polygonatum kingianum var. grandifolium, Polygonatum sibiricum Red, organic acid, BHLH transcription factor, bioinformatics family, identification