关键词: 铁皮石斛（Dendrobium officinale Kimura et Migo）, 根, 发育, 基因, 转录模式, 转录因子, 差异表达

Abstract: The Illumina HiSeq^TM4000 sequencing platform was used to sequence samples from two different parts of Dendrobium officinale roots （root tip and mature zone）, and GO clustering and KEGG analysis were performed on differentially expressed genes. The results showed that the high-quality reading length of Dendrobium officinale roots was assembled into 136 541 single genes with an average length of 982 bp. Identification and classification statistics were conducted on genes with the ability to encode transcription factors, and 2105 single genes were mapped to 55 categories of transcription factors. A total of 148 MADS boxes, 737 NACs, 449 WRKYs, 369 MYBs, and 105 ARFs were identified. The regulatory pathways of differentially expressed genes involved many pathways, including secondary metabolic pathways such as phenylpropanoid biosynthesis, environmental response pathways such as plant-pathogen interactions, and signal transduction pathways such as plant MAPK signaling pathway.

Key words: Dendrobium officinale Kimura et Migo, root, development, gene, transcriptional pattern, transcription factors, differential expression