湖北农业科学 ›› 2025, Vol. 64 ›› Issue (2): 6-12.doi: 10.14088/j.cnki.issn0439-8114.2025.02.002

• 资源·环境 • 上一篇    下一篇

不同栽培措施对雪茄烟根际土壤真菌和细菌群落结构及功能的影响

沈俊儒1, 和国优1,2, 唐旭兵1, 任龙辉1, 方保1, 甄安忠1, 孔垂思2   

  1. 1.云南香料烟有限责任公司,云南 保山 678000;
    2.云南省农业科学院农业环境资源研究所,昆明 650205
  • 收稿日期:2024-03-08 出版日期:2025-02-25 发布日期:2025-03-07
  • 通讯作者: 孔垂思,研究员,博士,主要从事土肥植保研究,(电子信箱)kcs@yaas.org.cn。
  • 作者简介:沈俊儒(1974-),男,湖北武汉人,助理研究员,博士,主要从事烟草标准化研究,(电子信箱)121831391@qq.com。
  • 基金资助:
    云南省烟草公司重大科技专项(2022530000241005)

Effects of different cultivation measures on the structure and function of fungal and bacterial communities in the rhizosphere soil of cigar tobacco

SHEN Jun-ru1, HE Guo-you1,2, TANG Xu-bing1, REN Long-hui1, FANG Bao1, ZHEN An-zhong1, KONG Chui-si2   

  1. 1. Yunnan Oriental Tobacco Co., Ltd., Baoshan 678000, Yunnan, China;
    2. Institute of Agricultural Environment and Resources, Yunnan Academy of Agricultural Sciences, Kunming 650205, China
  • Received:2024-03-08 Published:2025-02-25 Online:2025-03-07

摘要: 利用高通量测序技术研究雪茄烟不同栽培方式下根际土壤真菌和细菌群落在门、属水平上的变化,探讨栽培方式对根际真菌和细菌群落结构多样性的影响,并进行了功能基因预分析。结果表明,与常规栽培(CK)相比,其他栽培方式显著降低了根际土壤中真菌群落的丰富度(P<0.05),且明显降低了各多样性指数;栽培方式没有改变门水平上的真菌和细菌的主要组成,但对有益细菌的聚集有影响;根际土壤优势真菌门为子囊菌门(Ascomycota)和担子菌门(Basidiomycota),优势真菌属为镰刀菌属(Fusarium)、链格孢属(Alternaria)和青霉菌属(Penicillium);优势细菌门为变形菌门(Proteobacteria)和放线菌门(Actinobacteriota)(CK 除外),但不同栽培方式下细菌优势属各不相同。施用棘孢木霉菌或生物碳有机肥的处理对根际真菌和细菌群落的影响效果更明显。

关键词: 根际土壤, 栽培措施, 雪茄烟, 真菌群落, 细菌群落, 结构, 功能

Abstract: Using high-throughput sequencing technology, the changes of fungal and bacterial communities at the phylum and genus level in the rhizosphere soil under different cultivation methods of cigar tobacco were studied, the effects of cultivation methods on the structural diversity of fungal and bacterial communities were discussed, and functional gene pre-analysis was carried out. The results showed that the richness and diversity of fungal community in rhizosphere soil were significantly decreased under different cultivation patterns compared with the conventional cultivation (CK), and the difference of the former was statistically significant(P<0.05). Cultivation measures did not change the main composition of fungi and bacteria at phylum level, but could significantly recruit beneficial bacteria in the rhizosphere soil. The dominant fungal phyla in the rhizosphere soil were Ascomycota and Basidiomycota, and the dominant fungal genera were Fusarium, Alternaria and Penicillium. The dominant bacterial phyla were Proteobacteria and Actinobacteriota (except CK), but the dominant bacterial genera varied under different treatments. The effect of Trichoderma asperellum or bio-carbon organic fertilizer on rhizosphere fungi and bacteria communities was more obvious.

Key words: rhizosphere soil, cultivation measures, cigar tobacco, fungal community, bacterial community, structure, function

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