贝莱斯芽孢杆菌EA19替代部分氟唑菌酰羟胺在防治小麦赤霉病中的应用

doi:10.14088/j.cnki.issn0439-8114.2026.01.012

湖北农业科学 ›› 2026, Vol. 65 ›› Issue (1): 66-72.doi: 10.14088/j.cnki.issn0439-8114.2026.01.012

贝莱斯芽孢杆菌EA19替代部分氟唑菌酰羟胺在防治小麦赤霉病中的应用

王文肖^1,2, 陈婷婷¹, 阙亚伟¹, 薛敏峰¹, 倪浩翔³, 邓春林⁴, 杨立军¹, 李利³, 龚双军¹, 周华众⁴, 曾凡松¹

1.农业农村部华中作物有害生物综合治理重点实验室/农作物重大病虫草害防控湖北省重点实验室/湖北省农业科学院植保土肥研究所,武汉 430064;
2.湖北省植物保护总站,武汉 430070;
3.长江大学生命科学学院,湖北荆州 434025;
4.武汉科诺生物科技股份有限公司,武汉 430074

收稿日期:2025-10-23 出版日期:2026-01-25 发布日期:2026-02-10
通讯作者: 周华众(1970-),推广研究员,硕士,主要从事植保技术研究与推广工作,(电子信箱)897015023@qq.com。
作者简介:王文肖(1999-),女,河南周口人,在读硕士研究生,研究方向为植物保护,(电子信箱)w2674685344@163.com;曾凡松(1980-),男,副研究员,博士,主要从事小麦病害防控技术研究,(电子信箱)zengfansong2005@126.com。
基金资助:
国家重点研发计划项目(2022YFD1400105); 湖北省农业科技攻关项目(HBNYHXGG2023-3); 国家小麦产业技术体系项目(CARS-03)

Application of Bacillus velezensis EA19 to substitute part of pydiflumetofen in the control of Fusarium head blight

WANG Wen-xiao^1,2, CHEN Ting-ting¹, QUE Ya-wei¹, XUE Min-feng¹, NI Hao-xiang³, DENG Chun-lin⁴, YANG Li-jun¹, LI Li³, GONG Shuang-jun¹, ZHOU Hua-zhong⁴, ZENG Fan-song¹

1. Key Laboratory of Integrated Pest Management on Crops in Central China, Ministry of Agriculture and Rural Affairs/Hubei Key Laboratory of Integrated Management of Crop Diseases, Insects and Weeds/Institute of Plant Protection and Soil Fertilizer, Hubei Academy of Agricultural Sciences, Wuhan 430064, China;
2. Hubei Plant Protection Station, Wuhan 430070, China;
3. College of Life Science, Yangtze University, Jingzhou 434025, Hubei, China;
4. Wuhan Kernel Bio-tech Co., Ltd., Wuhan 430074, China

Received:2025-10-23 Published:2026-01-25 Online:2026-02-10

摘要/Abstract

摘要： 结合生物信息学分析、室内生物测定与田间药效试验,对生防菌剂贝莱斯芽孢杆菌(Bacillus velezensis)EA19与琥珀酸脱氢酶(SDH)抑制剂氟唑菌酰羟胺(Pyd)联用防治小麦赤霉病的效果进行评价。生物信息学分析显示,EA19菌株含有核黄素蛋白（FP）和铁硫蛋白（IP）2种SDH蛋白组件,但与镰孢菌的FP和IP亲缘关系较远,且缺乏与Pyd相结合的细胞色素b(Cytb)蛋白。在浓度为0.1~12.5 μg/mL时,Pyd对EA19菌株生长无显著影响。EA19发酵液原粉和Pyd对禾谷镰孢菌PH-1的EC₅₀分别为19.65 g/L和0.12 μg/mL。在EC₅₀剂量下,2种药剂按药液体积比6︰4和3︰7复配时,对PH-1菌丝的联合毒力最强,毒性比率均为1.48,增效作用显著。田间试验表明,将Pyd SC分别减量60%和30%,并与EA19 WP按上述比例联用,其防治效果均可达到与全量Pyd SC相当的水平。但对脱氧雪腐镰刀菌烯醇(DON)毒素防治效果显著低于全量Pyd SC。两个联用处理的小麦实际产量和千粒重与全量Pyd SC均无显著差异,其中Pyd SC减量30%与EA19 WP联用表现出提高千粒重的趋势。

关键词: 小麦赤霉病, 贝莱斯芽孢杆菌(Bacillus velezensis), 氟唑菌酰羟胺, 联用, 防治效果

Abstract: The efficacy of combining the biocontrol agent Bacillus velezensis EA19 with the succinate dehydrogenase inhibitor (SDHI) fungicide pydiflumetofen (Pyd) against Fusarium head blight (FHB) in wheat was evaluated through an integration of bioinformatics analysis, in vitro bioassays, and field efficacy trials. Bioinformatics analysis revealed that strain EA19 possessed two SDH components (flavoprotein FP and iron-sulfur protein IP), which were phylogenetically distant from their homologs in Fusarium spp., and lacked the cytochrome b (Cytb) protein target for Pyd. In vitro compatibility tests showed that Pyd concentrations ranging from 0.1 to 12.5 μg/mL had no significant inhibitory effect on EA19 growth. The EC₅₀ of EA19 fermentation broth powder and Pyd against Fusarium graminearum PH-1 were 19.65 g/L and 0.12 μg/mL, respectively. At their respective EC₅₀ doses, co-application of the two agents at solution volume ratios of 6︰4 and 3︰7 (EA19︰Pyd) yielded the strongest synergistic inhibition of PH-1 mycelial growth, with toxicity ratios of 1.48 for both combinations, indicating significant synergism. Field trials demonstrated that combining a 60% or 30% reduced dose of Pyd suspension concentrate (SC) with EA19 wettable powder (WP) at the aforementioned ratios achieved control efficacy statistically equivalent to the full dose of Pyd SC alone. However, the efficacy in reducing deoxynivalenol (DON) mycotoxin content was significantly lower than that of the full Pyd SC treatment. Both combination treatments resulted in wheat grain yield and thousand-kernel weight comparable to the full Pyd SC treatment. Notably, the treatment combining a 30% reduced Pyd SC dose with EA19 WP showed a tendency to increase thousand-kernel weight.

Key words: Fusarium head blight, Bacillus velezensis, pydiflumetofen, combination, control efficacy

中图分类号:

王文肖, 陈婷婷, 阙亚伟, 薛敏峰, 倪浩翔, 邓春林, 杨立军, 李利, 龚双军, 周华众, 曾凡松. 贝莱斯芽孢杆菌EA19替代部分氟唑菌酰羟胺在防治小麦赤霉病中的应用[J]. 湖北农业科学, 2026, 65(1): 66-72.

WANG Wen-xiao, CHEN Ting-ting, QUE Ya-wei, XUE Min-feng, NI Hao-xiang, DENG Chun-lin, YANG Li-jun, LI Li, GONG Shuang-jun, ZHOU Hua-zhong, ZENG Fan-song. Application of Bacillus velezensis EA19 to substitute part of pydiflumetofen in the control of Fusarium head blight[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(1): 66-72.

参考文献

[1] 马忠华,陈云,尹燕妮.小麦赤霉病流行成灾原因分析及防控对策探讨[J].中国科学基金,2020,34(4):464-469.
[2] MISHRA S,SRIVASTAVA S,DEWANGAN J,et al.Global occurrence of deoxynivalenol in food commodities and exposure risk assessment in humans in the last decade: A survey[J].Critical reviews in food science and nutrition,2020,60(8):1346-1374.
[3] GB/T2761—2022,食品安全国家标准食品中真菌毒素限量[S].
[4] 段亚冰,周明国.我国小麦赤霉病流行与化学防控现状及控病降毒关键技术理论创新与应用[J].现代农药,2024,23(2):13-21.
[5] 陈宏州,吴佳文,庄义庆,等.不同杀菌剂对小麦赤霉病及籽粒DON毒素的控制效果[J].植物保护,2021,47(6):307-317.
[6] LANCASTER C R D. Succinate: Quinone oxidoreductases: An overview[J].Biochimica et biophysica acta: BBA-bioenergetics,2002,1553(1-2):1-6.
[7] 魏阁,高梦琪,朱晓磊,等.靶向琥珀酸脱氢酶的酰胺类杀菌剂的研究进展[J].农药学学报,2019,21(S1):673-680.
[8] 杨绍华. 农抗“1465”的筛选及防治小麦赤霉病试验简报[J].福建稻麦科技,1983(3):38-41.
[9] 李宏伟,张扬,郑斐,等.5种杀菌剂对小麦赤霉病的活性测定方法比较[J].世界农药,2022,44(6):54-60.
[10] 毛玉帅,段亚冰,周明国,等.琥珀酸脱氢酶抑制剂类杀菌剂抗性研究进展[J].农药学学报,2022,24(5):937-948.
[11] SHAO W Y,WANG J R,WANG H Y,et al.Fusarium graminearum FgSdhC1 point mutation A78V confers resistance to the succinate dehydrogenase inhibitor pydiflumetofen[J].Pest management science,2022,78(5): 1780-1788.
[12] SUN H Y,CUI J H,TIAN B H,et al.Resistance risk assessment for Fusarium graminearum to pydiflumetofen, a new succinate dehydrogenase inhibitor[J].Pest management science,2020,76(4):1549-1559.
[13] ZHOU F,ZHOU H H,HAN A H,et al.Mechanism of pydiflumetofen resistance in Fusarium graminearum in China[J].Journal of fungi,2022,9(1):62.
[14] CHEN W C,WEI L L,ZHAO W C,et al.Resistance risk assessment for a novel succinate dehydrogenase inhibitor pydiflumetofen in Fusarium asiaticum[J].Pest management science,2021,77(1):538-547.
[15] PALAZZINI J,REYNOSO A,YERKOVICH N,et al.Combination of Bacillus velezensis RC218 and chitosan to control Fusarium head blight on bread and durum wheat under greenhouse and field conditions[J].Toxins, 2022,14(7):499.
[16] 刘悦,曾凡松,龚双军,等.解淀粉芽胞杆菌EA19菌株对小麦赤霉病的防治效果[J].植物保护学报,2020,47(6):1270-1276.
[17] 陈文华,殷宪超,武德亮,等.小麦赤霉病生物防治研究进展[J].江苏农业科学,2020,48(4):12-18.
[18] 黄慧婧,罗坤.芽孢杆菌与杀菌剂复配防治植物病害的研究进展[J].微生物学通报,2021,48(3):938-947.
[19] 王文肖,刘美玲,阙亚伟,等.贝莱斯芽孢杆菌EA19与多菌灵复配防治小麦赤霉病研究[J].河南农业科学,2024,53(10):117-126.
[20] 张震,邱海萍,柴荣耀,等.一株小麦赤霉病生防菌的鉴定及其生防机制初探[J].中国生物防治学报,2022,38(3):673-680.
[21] 毛雪琴,彭志荣,邱海萍,等.生防菌株MT-06发酵条件及复配杀菌剂对小麦赤霉病的防效[J].浙江农业科学,2013,54(7):821-824.
[22] 曾凡松,向礼波,杨立军,等.一株内生细菌EA19的分离鉴定及其对小麦白粉病菌的抑制效果[J].湖北农业科学,2012,51(23):5344-5347.
[23] ZENG F S,YUAN B,SHI W Q,et al.Complete genome sequence of Bacillus amyloliquefaciens EA19, an endophytic bacterium with biocontrol potential isolated from Erigeron annuus[J].Microbiology resource announcements,2021,10(39):e00753-21.
[24] NY/T2293.1—2012,细菌微生物农药枯草芽孢杆菌第1部分:枯草芽孢杆菌母药[S].
[25] 张宗炳. 杀虫药剂的毒力测定——原理·方法·应用[M].北京:科学出版社,1988.396-398.
[26] HALL B G.Building phylogenetic trees from molecular data with MEGA[J].Molecular biology and evolution, 2013,30(5):1229-1235.
[27] 国家药典委员会.中华人民共和国药典三部 2020年版[M].北京:中国医药科技出版社,2020.219-224.
[28] 黄国洋. 农药试验技术与评价方法[M].北京:中国农业出版社,2000.30-31.
[29] 杨胜雨,杨飞,段海明,等.生防菌代谢物与杀菌剂复配对花生褐斑病菌的抑制活性[J].江汉大学学报(自然科学版),2023,51(2):61-67.
[30] NY/T1464.15—2007,农药田间药效试验准则第15部分:杀菌剂防治小麦赤霉病[S].
[31] ZHU X L,XIONG L,LI H,et al.Computational and experimental insight into the molecular mechanism of carboxamide inhibitors of succinate-ubquinone oxidoreductase[J].Chem Med Chem,2014, 9(7):1512-1521.
[32] 赵雅,张岱,杨志辉,等.贝莱斯芽胞杆菌HN-Q-8菌株发酵液稳定性测定及抑菌活性成分分析[J].微生物学通报,2020,47(2):490-499.
[33] HANIF A,ZHANG F,LI P P,et al.Fengycin produced by Bacillus amyloliquefaciens FZB42 inhibits Fusarium graminearum growth and mycotoxins biosynthesis[J].Toxins,2019,11(5):295.
[34] 毕秋艳,韩秀英,马志强,等.枯草芽胞杆菌HMB-20428与化学杀菌剂互作对葡萄霜霉病菌抑制作用和替代部分化学药剂减量用药应用[J].植物保护学报,2018,45(6):1396-1404.
[35] 王叶青,刘芳,潘纪源,等.巨大芽孢杆菌与噁霉灵联用对甜瓜连作障碍的缓解效果[J].农药学学报,2022,24(4):762-770.

贝莱斯芽孢杆菌EA19替代部分氟唑菌酰羟胺在防治小麦赤霉病中的应用

Application of Bacillus velezensis EA19 to substitute part of pydiflumetofen in the control of Fusarium head blight

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