两株生防芽孢杆菌对烟草青枯病的防治效果

doi:10.14088/j.cnki.issn0439-8114.2026.02.014

湖北农业科学 ›› 2026, Vol. 65 ›› Issue (2): 95-100.doi: 10.14088/j.cnki.issn0439-8114.2026.02.014

两株生防芽孢杆菌对烟草青枯病的防治效果

程伟¹, 王辽源¹, 霍光², 孔伟³, 施河丽², 向必坤², 张冉¹, 董睿婕¹, 杨兆钰¹, 曹媛媛¹, 谭军², 王瑞⁴

1.安徽农业大学生命科学学院,合肥 230036;
2.湖北省烟草公司恩施州公司,湖北恩施 445000;
3.中国烟草总公司湖北省公司,武汉 430030;
4.湖北省烟草科学研究院,武汉 430030

收稿日期:2025-10-20 出版日期:2026-03-04 发布日期:2026-03-04
通讯作者: 谭军(1970-),男,湖北恩施人,高级农艺师,主要从事烟草栽培、绿色防控研究,(电子信箱)tanjun20713@163.com。
作者简介:程伟(2001-),男,安徽黄山人,在读硕士研究生,研究方向为微生物与植物互作,(电子信箱)2668830874@qq.com;共同第一作者,王辽源(1998-),男,安徽宿州人,硕士,主要从事微生物与植物互作研究,(电子信箱)wangliaoyuan2023@163.com;王瑞(1975-),男,湖北恩施人,研究员,博士,主要从事烟草栽培、病虫害防治研究,(电子信箱)wangrui2999@126.com。
基金资助:
湖北省烟草公司科技项目(027Y2022-007)

The biocontrol efficacy of two Bacillus strains against tobacco bacterial wilt

CHENG Wei¹, WANG Liao-yuan¹, HUO Guang², KONG Wei³, SHI He-li², XIANG Bi-kun², ZHANG Ran¹, DONG Rui-jie¹, YANG Zhao-yu¹, CAO Yuan-yuan¹, TAN Jun², WANG Rui⁴

1. School of Life Sciences, Anhui Agricultural University, Hefei 230036, China;
2. Enshi Branch of Hubei Provincial Tobacco Company, Enshi 445000, Hubei, China;
3. Hubei Tobacco Company Limited, China National Tobacco Corporation, Wuhan 430030, China;
4. Tobacco Research Institute of Hubei Province, Wuhan 430030, China

Received:2025-10-20 Published:2026-03-04 Online:2026-03-04

摘要/Abstract

摘要： 为评估萎缩芽孢杆菌(Bacillus atrophaeus)BS17和贝莱斯芽孢杆菌(Bacillus velezensis)BS22对烟草青枯病的防治效果并确定最佳施用时期,采用LIVE/DEAD荧光染色及扫描电镜观察两菌株对青枯劳尔氏菌（Ralstonia solanacearum）的抑菌作用,分析其抑菌机制,并通过盆栽试验在播种期、出苗期和移栽期分别施用菌液,比较不同时期的防治效果。结果表明,两菌株主要通过破坏青枯病病原菌青枯劳尔氏菌细胞膜实现抑菌作用。BS22在播种期施用防治效果最佳,达98.23%,显著优于BS17(79.64%,P<0.001);出苗期施用两菌株防治效果无显著差异(BS17:88.49%,BS22:85.84%);移栽期施用防治效果均显著降低(BS17:3.54%,BS22:48.67%)。BS22菌株对烟草青枯病的防治效果优于BS17,尤其在播种期施用效果最佳。

关键词: 萎缩芽孢杆菌(Bacillus atrophaeus)BS17, 贝莱斯芽孢杆菌(Bacillus velezensis)BS22, 烟草青枯病, 防治效果, 施用时期

Abstract: To evaluate the biocontrol efficacy of Bacillus atrophaeus strain BS17 and Bacillus velezensis strain BS22 against tobacco bacterial wilt and determine the optimal application timing, LIVE/DEAD fluorescence staining and scanning electron microscopy were employed to observe the inhibitory effects of these two strains on Ralstonia solanacearum (the causal agent of bacterial wilt) and analyze their inhibitory mechanisms. Additionally, pot experiments were conducted with bacterial suspensions applied at three key growth stages: Sowing stage, seedling emergence stage, and transplanting stage. The results demonstrated that both strains primarily inhibit Ralstonia solanacearum by disrupting the integrity of the bacterial cell membrane. The BS22 strain exhibited superior control efficacy when applied at the sowing stage (98.23%), significantly higher than that of BS17 (79.64%, P<0.001). When applied at the seedling emergence stage, no significant difference in control efficacy was observed between the two strains (BS17: 88.49%, BS22: 85.84%). However, when applied at the transplanting stage, both strains exhibited significantly reduced control efficacy (BS17: 3.54%, BS22: 48.67%). The BS22 strain demonstrated superior biocontrol efficacy against tobacco bacterial wilt compared to the BS17 strain, particularly when applied at the sowing stage.

Key words: Bacillus atrophaeus strain BS17, Bacillus velezensis strain BS22, tobacco bacterial wilt, control efficacy, application timing

中图分类号:

程伟, 王辽源, 霍光, 孔伟, 施河丽, 向必坤, 张冉, 董睿婕, 杨兆钰, 曹媛媛, 谭军, 王瑞. 两株生防芽孢杆菌对烟草青枯病的防治效果[J]. 湖北农业科学, 2026, 65(2): 95-100.

CHENG Wei, WANG Liao-yuan, HUO Guang, KONG Wei, SHI He-li, XIANG Bi-kun, ZHANG Ran, DONG Rui-jie, YANG Zhao-yu, CAO Yuan-yuan, TAN Jun, WANG Rui. The biocontrol efficacy of two Bacillus strains against tobacco bacterial wilt[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(2): 95-100.

参考文献

[1] 何晓冰,黄昆鹏,李俊营,等.烟草镰刀菌根腐病根际土壤微生物群落结构及多样性[J].浙江农业科学,2025, 66(2):411-420.
[2] MANSFIELD J,GENIN S,MAGORI S,et al.Top 10 plant pathogenic bacteria in molecular plant pathology[J].Molecular plant pathology,2012,13(6):614-629.
[3] SHI H Q,JIANG J L,YU W,et al.Naringenin restricts the colonization and growth of Ralstonia solanacearum in tobacco mutant KCB-1[J]. Plant physiology, 2024, 195(3): 1818-1834.
[4] LI J Y, ZHAO Q Q, WURIYANGHAN H, et al.Biocontrol bacteria strains Y4 and Y8 alleviate tobacco bacterial wilt disease by altering their rhizosphere soil bacteria community[J]. Rhizosphere, 2021, 19:100390.
[5] LI L C, FENG X J, TANG M, et al.Antibacterial activity of Lansiumamide B to tobacco bacterial wilt (Ralstonia solanacearum)[J]. Microbiological research, 2014, 169(7-8): 522-526.
[6] 何洪令,李钠钾,孙成成,等.烟草青枯病的生物防治研究进展[J]. 植物医生, 2021, 34(2): 4-8.
[7] 陈继峰,赵松辉,蔡凯旋,等. 河南烟草连作状况调查及连作障碍解决对策研究[J]. 中国园艺文摘, 2014, 30(5): 218-222.
[8] ÓBRIEN P A.Biological control of plant diseases[J]. Australasian plant pathology, 2017, 46(4): 293-304.
[9] AHMAD F, AHMAD I, KHAN M S.Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities[J]. Microbiological research, 2008, 163(2): 173-181.
[10] 郝浩浩,张雨薇,唐培培,等.生防菌防治烟草黑胫病的研究进展[J].农学学报, 2025, 15(5): 41-47.
[11] KASHYAP A S, MANZAR N, MESHRAM S, et al.Screening microbial inoculants and their interventions for cross-kingdom management of wilt disease of solanaceous crops- a step toward sustainable agriculture[J]. Frontiers in microbiology, 2023, 14: 1174532.
[12] LI K, ZHANG X, CHEN Y, et al.A promising biocontrol agent of Bacillus velezensis VC3 against Magnaporthe oryzae and Colletotrichum gloeosporioides in Plants[J]. Processes, 2024, 12(7): 1490.
[13] DONG Q Q, LIU Q X, GOODWIN P H, et al.Isolation and genome-based characterization of biocontrol potential of Bacillus Siamensis YB-1631 against wheat crown rot caused by Fusarium Pseudograminearum[J]. Journal of fungi, 2023, 9(5): 547.
[14] YAMAMOTO S, SHIRAISHI S, KAWAGOE Y, et al.Impact of Bacillus amyloliquefaciens S13-3 on control of bacterial wilt and powdery mildew in tomato[J]. Pest management science, 2015, 71(5): 722-727.
[15] LI E F, LI Y X, DAI XY, et al.Identification of two Bacillus strains with antimicrobial activity and preliminary evaluation of their biocontrol efficiency[J]. Horticulturae, 2022, 8(8): 744.
[16] LAM V, MEYER T, ARIAS A A, et al.Bacillus cyclic lipopeptides iturin and fengycin control rice blast caused by Pyricularia oryzae in potting and acid sulfate soils by direct antagonism and induced systemic resistance[J]. Microorganisms, 2021, 9(7): 1441.
[17] RUAN Y N, NONG C H, JINTRAWET A, et al.A smooth vetch (Vicia villosa var.) strain endogenous to the broad-spectrum antagonist Bacillus siamensis JSZ06 alleviates banana wilt disease[J]. Frontiers in plant science, 2024, 15: 1410197.
[18] WU K, YUAN S F, XUN G H, et al.Root exudates from two tobacco cultivars affect colonization of Ralstonia solanacearum and the disease index[J]. European journal of plant pathology, 2015, 141(4): 667-677.
[19] YUAN J, ZHANG N, HUANG Q W, et al.Organic acids from root exudates of banana help root colonization of PGPR strain Bacillus amyloliquefaciens NJN-6[J]. Scientific reports, 2015, 5: 13438.

两株生防芽孢杆菌对烟草青枯病的防治效果

The biocontrol efficacy of two Bacillus strains against tobacco bacterial wilt

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