基于芬芥素合成的解淀粉芽孢杆菌内源启动子筛选与功能验证

doi:10.14088/j.cnki.issn0439-8114.2026.02.031

湖北农业科学 ›› 2026, Vol. 65 ›› Issue (2): 209-214.doi: 10.14088/j.cnki.issn0439-8114.2026.02.031

基于芬芥素合成的解淀粉芽孢杆菌内源启动子筛选与功能验证

周璐, 田缘, 于冲, 夏海华, 闫更轩

黑龙江省科学院微生物研究所,哈尔滨 150010

收稿日期:2025-09-08 出版日期:2026-03-04 发布日期:2026-03-04
通讯作者: 闫更轩（1996-）,男,黑龙江哈尔滨人,助理研究员,硕士,主要从事微生物药物学研究,（电话）18745058190（电子信箱）predawnyan@126.com。
作者简介:周璐（2000-）,女,黑龙江哈尔滨人,研究实习员,硕士,主要从事微生物学研究,（电话）15645161317（电子信箱）154399341@qq.com。
基金资助:
黑龙江省科学院杰出青年基金项目（2023QNJJ003）

Screening and functional validation of endogenous promoters from Bacillus amyloliquefaciens for fengycin synthesis

ZHOU Lu, TIAN Yuan, YU Chong, XIA Hai-hua, YAN Geng-xuan

Institute of Microbiology, Heilongjiang Academy of Sciences, Harbin 150010, China

Received:2025-09-08 Published:2026-03-04 Online:2026-03-04

摘要/Abstract

摘要： 以解淀粉芽孢杆菌(Bacillus amyloliquefaciens)TF28为宿主,结合基因组与转录组数据,筛选出12个高表达基因的上游内源启动子。通过构建GFP报告系统,在芽孢杆菌中评估各启动子活性,发现PspoVG、PqoxC、PqoxB和Pgpd的启动强度高于常用启动子P43。在PspoVG启动子驱动下,fenC基因的转录水平是P43启动子的4.62倍,芬芥素产量从84.3 mg/L提高至101.2 mg/L。在启动子结构方面,PspoVG的-35区和-10区序列与典型强启动子的保守序列高度一致,这可能是其高效驱动转录的结构基础。

关键词: 芬芥素, 解淀粉芽孢杆菌(Bacillus amyloliquefaciens), 内源启动子, 筛选, 功能验证

Abstract: Using Bacillus amyloliquefaciens TF28 as the host, and by integrating genomic and transcriptomic data, 12 upstream endogenous promoters from highly expressed genes were screened. By constructing a GFP reporter system and evaluating the activity of each promoter in Bacillus, it was found that the promoter strengths of PspoVG, PqoxC, PqoxB, and Pgpd were higher than that of the commonly used promoter P43. When driven by the PspoVG promoter, the transcriptional level of the fenC gene was 4.62 times that of the P43 promoter, and the fengycin yield increased from 84.3 mg/L to 101.2 mg/L. Regarding promoter structure, the sequences of the -35 and -10 regions of PspoVG were highly consistent with the consensus sequences of typical strong promoters, which might be the structural basis for its high-efficiency transcriptional drive.

Key words: fengycin, Bacillus amyloliquefaciens, endogenous promoter, screening, functional validation

中图分类号:

Q933

周璐, 田缘, 于冲, 夏海华, 闫更轩. 基于芬芥素合成的解淀粉芽孢杆菌内源启动子筛选与功能验证[J]. 湖北农业科学, 2026, 65(2): 209-214.

ZHOU Lu, TIAN Yuan, YU Chong, XIA Hai-hua, YAN Geng-xuan. Screening and functional validation of endogenous promoters from Bacillus amyloliquefaciens for fengycin synthesis[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(2): 209-214.

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基于芬芥素合成的解淀粉芽孢杆菌内源启动子筛选与功能验证

Screening and functional validation of endogenous promoters from Bacillus amyloliquefaciens for fengycin synthesis

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