纳米氧化铜对辣椒种子发芽、光合特性及抗氧化酶活性的影响

doi:10.14088/j.cnki.issn0439-8114.2026.08.016

摘要/Abstract

摘要： 为探究纳米氧化铜对作物生长及生理生化指标的影响,以辣椒(Capsicum annuum L.)作为试验材料,设置5个处理：CK(0 mg/L)、A1(10 mg/L)、A2(20 mg/L)、A3(50 mg/L)、A4(100 mg/L),测定不同浓度纳米氧化铜溶液处理下辣椒的发芽率、辣椒叶片的光合特性及抗氧化酶活性等生理生化指标,并观察不同浓度溶液处理下辣椒胚根的细胞骨架。结果表明,不同浓度的纳米氧化铜溶液浸泡对辣椒的发芽有抑制作用,其中以A3处理效果最为显著,与CK相比,发芽率降低了25.6个百分点;不同浓度纳米氧化铜溶液浇灌对辣椒的净光合速率具有促进作用,其中以A4效果最好,比CK增加了50.4%;不同浓度纳米氧化铜溶液浇灌可提高辣椒叶片组织中NAD-MDH、CAT、SOD、POD活性,其中以A1处理时NAD-MDH活性提升效果最为显著,以A3处理时CAT、SOD、POD活性提升程度最大;不同浓度纳米氧化铜溶液浸泡会对辣椒种子胚根的细胞骨架造成一定影响,其中A3处理下,细胞皮层肌动蛋白丝信号增强,应力纤维增多、变粗。

关键词: 纳米氧化铜, 辣椒(Capsicum annuum L.), 细胞骨架, 光合特性, 抗氧化酶, 生长发育

Abstract: To investigate the effects of nano-copper oxide (nano-CuO) on crop growth and physiological-biochemical characteristics, pepper (Capsicum annuum L.) was used as the test material. Five treatments were set up: CK(0 mg/L), A1(10 mg/L), A2(20 mg/L), A3 (50 mg/L), and A4 (100 mg/L). Various physiological and biochemical indicators were measured under different concentrations of nano-CuO solution, including seed germination rate, photosynthetic characteristics of pepper leaves, and antioxidant enzyme activities. Additionally, the cytoskeleton of pepper radicle cells was observed under different concentrations of nano-CuO solution. The results showed that soaking pepper seeds with different concentrations of nano-CuO solution had an inhibitory effect on seed germination, with the most significant effect observed in the A3 treatment. Compared with CK, the germination rate decreased by 25.6 percentage points. Irrigating pepper plants with different concentrations of nano-CuO solution promoted the net photosynthetic rate (Pn), with the best effect observed in the A4 treatment, where Pn increased by 50.4% compared with CK. Irrigation with different concentrations of nano-CuO solution also enhanced the activities of NAD-MDH, CAT, SOD, and POD enzymes in pepper leaf tissues. The most significant increase in NAD-MDH activity was observed in the A1 treatment, and the greatest increases in CAT, SOD, and POD activities were observed in the A3 treatment. Soaking pepper seeds with different concentrations of nano-CuO solution exerted certain effects on the cytoskeleton of pepper radicle cells. Specifically, under the A3 treatment, cortical actin filament signals in the cells were enhanced, and stress fibers became more numerous and thicker.

Key words: nano-copper oxide, pepper(Capsicum annuum L.), cytoskeleton, photosynthetic characteristics, antioxidant enzymes, growth and development

中图分类号:

张华鑫, 江恪诺, 陈晓峰. 纳米氧化铜对辣椒种子发芽、光合特性及抗氧化酶活性的影响[J]. 湖北农业科学, 2026, 65(8): 105-110.

ZHANG Hua-xin, JIANG Ke-nuo, CHEN Xiao-feng. Effects of nano-copper oxide on seed germination, photosynthetic characteristics, and antioxidant enzyme activities of pepper[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(8): 105-110.

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