月季优质品系的高效繁殖体系研究

doi:10.14088/j.cnki.issn0439-8114.2022.01.023

湖北农业科学 ›› 2022, Vol. 61 ›› Issue (1): 119-121.doi: 10.14088/j.cnki.issn0439-8114.2022.01.023

月季优质品系的高效繁殖体系研究

杨海牛¹, 何超超², 宁国贵², 沙飞¹

1.武汉市花木有限公司,武汉 430000;
2.华中农业大学园艺林学学院,武汉 430070

收稿日期:2021-05-17 出版日期:2022-01-10 发布日期:2022-01-26
通讯作者: 沙飞（1977-）,男,湖北钟祥人,高级工程师,主要从事园林植物新品种繁育及推广应用工作,（电话）13871308906（电子信箱）caigoubu1977@163.com。
作者简介:杨海牛（1980-）,男,湖北南漳人,高级工程师,主要从事园林植物新品种繁育及推广应用工作,（电话）13886189281（电子信箱）wuhanhuamu1979@163.com。
基金资助:
校企（武汉市花木有限公司+华中农业大学）合作项目（0220180024）

Study on high efficient propagation system of Rosa chinensis ‘Red and Pink’

YANG Hai-niu¹, HE Chao-chao², NING Guo-gui², SHA Fei¹

1. Wuhan Flower-wood Limited Company,Wuhan 430000,China;
2. College of Horticultural and Forestry Sciences, Huazhong Agriculture University,Wuhan 430070,China

Received:2021-05-17 Online:2022-01-10 Published:2022-01-26

摘要/Abstract

摘要： 以古老月季优质品系的小叶及复叶柄为外植体,探讨了月月粉（Rosa chinensis ‘Pink China’）自交后代一优良品系月月红粉（Rosa chinensis ‘Red and Pink’）的愈伤组织诱导、体细胞胚胎发生与增殖及植株高效再生的方法。结果表明,2,4-D可能是月月红粉最适宜愈伤诱导的生长激素;最佳外植体为复叶柄;同时添加L-p和AgNO₃对体细胞胚发生有促进作用;在MS+（3.0,4.0,5.0,6.0 mg/L）2,4-D+400 mg/L L-p+10 mg/L AgNO₃+3% Glucose+3 g/L GEL培养基上,外植体最初形成愈伤组织,经过16周培养后均不同程度发生体细胞胚;适合月月红粉体细胞胚增殖的培养基为：MS+1.0 mg/L 2,4-D+0.05 mg/L BA+3% Glucose+0.3% GEL。体细胞胚在萌发培养基1/2 MS+3% Glucose+0.3% GEL+1.0 mg/ L BA+0.01 mg/L NAA上萌发培养,最终建立了以月月红粉体细胞胚发生途径为基础的高效再生体系,为加快蔷薇属植物的功能基因验证及相应月季分子育种进度奠定了基础。

关键词: 月月红粉（Rosa chinensis ‘Red and Pink’）, 胚性愈伤组织, 体细胞胚发生, 增殖, 再生

Abstract: This study describes an effective regeneration system via calli induction, somatic embryogenesis and embryos germination using leaflets and compound leaf as explants in Rosa chinensis ‘Red and Pink’. The results showed that, high-frequency primary somatic embryos inductions were obtained from petioles of compound leaf combining 2, 4-D, that L-p and as AgNO₃ promoted somatic embryogenesis. When explants were incubated on MS medium supplemented with various concentration of 2, 4-D （3.0, 4.0, 5.0, 6.0 mg/L） respectively, added 400 mg/L L-p and 10 mg/L AgNO₃ and solidified using 0.3% Gel, after 16 weeks culture, they firstly formed embryogenic calli and finally somatic embryos. MS medium containing 1.0 mg/L 2, 4-D, 0.05 mg/L 6- BA and 3% glucose was found to be the most effective in the proliferation of somatic embryos. After germination culture on medium（1/2 MS+3% Glucose+0.3% GEL+1.0 mg/L BA+0.01 mg/L NAA）, a somatic embryogenesis based regeneration protocol were developed and it will be significant to accelerate the propagation and extensive utilization of Rosa genus species.

Key words: Rosa chinensis ‘Red and Pink’, embryogenic callus, somatic embryogenesis, propagation, regeneration

中图分类号:

S685.12

杨海牛, 何超超, 宁国贵, 沙飞. 月季优质品系的高效繁殖体系研究[J]. 湖北农业科学, 2022, 61(1): 119-121.

YANG Hai-niu, HE Chao-chao, NING Guo-gui, SHA Fei. Study on high efficient propagation system of Rosa chinensis ‘Red and Pink’[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(1): 119-121.

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月季优质品系的高效繁殖体系研究

Study on high efficient propagation system of Rosa chinensis ‘Red and Pink’

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