关键词: 鸡枞菌（Termitomyces albuminosus Heim）, 菌圃, 近周土壤, 宏基因组测序, 细菌群落

Abstract: In order to understand the symbiotic relationship between Termitomyces albuminosus Heim and other microorganisms, metagenomic sequencing was performed on the fungal combs of Termitomyces albuminosus and their surrounding soils collected from the Qianxinan Buyi and Miao Autonomous Prefecture of Guizhou Province using the Illumina Novaseq sequencing platform, and the bacterial community structure and diversity of Termitomyces albuminosus Heim combs and their surrounding soil were studied by bioinformatics analysis. The results showed that at the phylum level, the dominant bacterial communities were Proteobacteria, Acidobacteria, Actinobacteria, Bacteroidetes, and Chloroflexi. At the class level, Alphaproteobacteria was predominant, with a higher abundance in middle-layer soil samples of surrounding soils than in other samples. The relative abundances of Betaproteobacteria and Gammaproteobacteria in nest cavity walls were also significantly higher than those in other samples. Alpha diversity results indicated that bacterial diversity in nest cavity walls and comb soils was lower than that in surrounding soils. Redundancy analysis (RDA) revealed that latitude and altitude exerted the greatest effects on soil microbial community structure. The contribution rates of environmental factors in descending order were latitude, altitude, longitude, and pH.

Key words: Termitomyces albuminosus Heim, fungal comb, surrounding soil, metagenome sequencing, bacterial community