湖北农业科学 ›› 2019, Vol. 58 ›› Issue (22): 111-113.doi: 10.14088/j.cnki.issn0439-8114.2019.22.025

• 植物保护 • 上一篇    下一篇

苏云金芽孢杆菌以色列亚种(Bti)发酵液的浓缩工艺研究

陈伟, 廖先清, 刘芳, 饶犇, 周荣华   

  1. 湖北省生物农药工程研究中心,武汉 430064
  • 收稿日期:2019-08-01 出版日期:2019-11-25 发布日期:2019-12-09
  • 通讯作者: 饶犇(1983-),男,湖北孝感人,助理研究员,博士,主要从事发酵工程方面的研究,(电话)15623076968(电子信箱)623253512@qq.com。
  • 作者简介:陈 伟(1969-),男,广东汕头人,高级农艺师,主要从事微生物农药研究与开发工作,(电话)027-59101932(电子信箱)258951482@qq.com。
  • 基金资助:
    国家重点研发计划项目(2017YFD0201205)

Studies on concentration process of BTi fermentation broth

CHEN Wei, LIAO Xian-qing, LIU Fang, RAO Ben, ZHOU Rong-hua   

  1. Hubei of Biopesticide Engineering Research Center,Wuhan 430064,China
  • Received:2019-08-01 Online:2019-11-25 Published:2019-12-09

摘要: 通过对苏云金芽孢杆菌以色列亚种(Bacillus thuringiensis subsp. israelensis,Bti)发酵液浓缩工艺的研究,探索了不同温度对浓缩液有效成分的影响程度,比较了不同浓缩方式、不同干燥方式处理下有效成分的回收率。结果表明,采用真空薄膜浓缩方式最终得到的产物质量是离心浓缩方式得到产物质量的176%,效价回收率提高了36%。

关键词: 苏云金芽孢杆菌以色列亚种(Bacillus thuringiensis subsp. israelensis), 真空薄膜浓缩, 离心浓缩

Abstract: Through the study on the concentration process of Bacillus thuringiensis subsp. israelensis(Bti) fermentation broth, the effects of different temperatures on the active components of the concentrate were explored. The recovery rates of the active components under different concentration methods and different drying methods were compared. The results show that the quality of the product finally obtained by the vacuum film concentration method is 176% of the mass of the product obtained by centrifugal concentration, and the recovery rate of the potency is increased by 36%.

Key words: Bacillus thuringiensis subsp. israelensis, vacuum film concentration, centrifugal concentration

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