湖北农业科学 ›› 2021, Vol. 60 ›› Issue (3): 70-74.doi: 10.14088/j.cnki.issn0439-8114.2021.03.013

• 园艺·特产 • 上一篇    下一篇

外源5-氨基乙酰丙酸对干旱胁迫下黄瓜种子萌发及幼苗生长的影响

邓硕真, 刘惠军, 王洪芹, 王红艳, 张倩茹, 张梅   

  1. 中国农业大学烟台研究院,山东 烟台 264670
  • 收稿日期:2020-03-31 发布日期:2021-02-25
  • 通讯作者: 张梅(1970-),女,副教授,硕士,主要从事植物生理学及植物抗逆性研究,(电话)18660586236(电子信箱)hjkx2006@sina.com。
  • 作者简介:邓硕真(1998-),女,山东德州人,在读本科生,研究方向为设施农业科学与工程,(电话)17852352336(电子信箱)17852352336@163.com
  • 基金资助:
    烟台市科技攻关项目(2017ZH097); 中国农业大学烟台研究院重点资助项目(YT201901); 大学生科研训练项目(U20183021)

Effects of exogenous 5-aminolevulinic acid on seed germination and seedling growth of cucumber under drought stress

DENG Shuo-zhen, LIU Hui-jun, WANG Hong-qin, WANG Hong-yan, ZHANG Qian-ru, ZHANG Mei   

  1. Yantai Institute, China Agricultural University,Yantai 264670, Shandong, China
  • Received:2020-03-31 Published:2021-02-25

摘要: 以津耘五号黄瓜(Cucumis sativus L.)为试验材料,以20% PEG-6000模拟干旱胁迫,研究不同浓度外源5-氨基乙酰丙酸(5-ALA)对干旱环境下黄瓜种子发芽及幼苗生长的影响。结果表明,20% PEG-6000模拟的干旱严重抑制了黄瓜种子的萌发,不同浓度的5-ALA可以缓解干旱胁迫对黄瓜种子萌发的影响,随着5-ALA浓度的升高,种子的发芽率、发芽指数、活力指数出现了先上升后下降的趋势,其中0.5 mg/L为最佳浓度。20% PEG-6000模拟的干旱抑制了黄瓜幼苗的生长发育,适宜浓度的5-ALA可以显著提高黄瓜幼苗的抗旱性,其中0.5 mg/L 5-ALA处理下,黄瓜幼苗的干重、壮苗指数、根系活力、可溶性糖含量、游离脯氨酸含量、总叶绿素含量、类胡萝卜素含量分别比干旱环境下提高99.13%、104.51%、70.42%、40.79%、163.46%、38.59%、126.92%。

关键词: 黄瓜(Cucumis sativus L.), 外源5-氨基乙酰丙酸, 种子萌发, 干旱胁迫

Abstract: Taking Jinyun NO.5 cucumber(Cucumis sativus L.) as the test materials, the experiment was conducted to study the effects of different concentrations 5-aminolevulinic acid(5-ALA) on seed germination and seedling growth of cucumber under the condition of 20% PEG-6000 drought stress. The results showed that, drought environment seriously inhibited the germination of cucumber seeds, and different concentrations of 5-ALA can alleviate the effects of drought stress on cucumber seed germination. With the increase of 5-ALA concentration, the germination rate, germination index and vigor index of seeds first increased and then decreased. Among them, 0.5 mg/L is the best concentration. Drought environment inhibits the growth of cucumber seedlings. Appropriate concentration of 5-ALA can significantly increase the drought resistance of cucumber seedlings. At a concentration of 0.5 mg/L, the dry weight, strong seedling index, root activity, soluble sugar content, free proline content, total chlorophyll content and carotenoid content of cucumber seedlings respectively increased by 99.13%, 104.51%, 70.42%, 40.79%, 163.46%, 38.59%, 126.92% compared with the dry environment.

Key words: cucumber(Cucumis sativus L.), 5-aminolevulinic acid, seed germination, drought stress

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