耐重金属肠杆菌GL-2鉴定及对小麦促生作用的研究

doi:10.14088/j.cnki.issn0439-8114.2021.23.010

湖北农业科学 ›› 2021, Vol. 60 ›› Issue (23): 44-48.doi: 10.14088/j.cnki.issn0439-8114.2021.23.010

耐重金属肠杆菌GL-2鉴定及对小麦促生作用的研究

蔡高磊^1,2, 周炀², 赵昌松², 彭宣和², 张振², 赵建宁^1,3

1.农业农村部农产品质量安全环境因子控制重点实验室,天津 300191;
2.丹江口库区十堰生态农业研究院,湖北十堰 442000;
3.农业农村部环境保护科研监测所,天津 300191

收稿日期:2021-04-15 出版日期:2021-12-10 发布日期:2021-12-21
通讯作者: 赵建宁（1981-）,男,副研究员,博士,主要从事生物多样性与生态农业、转基因作物环境安全评价研究,（电子信箱）zhaojianning@caas.cn。
作者简介:蔡高磊（1982-）,男,山东巨野人,高级农艺师,硕士,主要从事土壤微生物与生态保护研究,（电话）18772781917（电子信箱）caigaolei@163.com;
基金资助:
农业农村部产地环境污染防控重点实验室/天津市农业环境与农产品安全重点实验室开放课题基金项目（18nybcdhj-6）; 中国农业科学院科技创新工程协同创新任务（CAAS-XTCX2016015）

Identification on heavy metal-resistant Enterobacter ludwigii GL-2 and effects on growth of wheat

CAI Gao-lei^1,2, ZHOU Yang², ZHAO Chang-song², PENG Xuan-he², ZHANG zhen², ZHAO Jian-ning^1,3

1. Key Laboratory for Environmental Factors Control of Agro-product Quality Safety,Ministry of Agriculture and Rural Affaris,Tianjin 300191,China;
2. Danjiangkou Reservoir Shiyan Ecological Agriculture Institute,Shiyan 442000,Hubei,China;
3. Agro-Environmental Protection Institute,Ministry of Agriculture and Rural Affairs,Tianjin 300191,China

Received:2021-04-15 Online:2021-12-10 Published:2021-12-21

摘要/Abstract

摘要： 为分离出具有重金属抗性的植物根际促生菌,用添加了Cd的LB培养基和嗜铁素培养基筛选合适的菌株,用平板稀释法测定GL-2菌株的最低抑制浓度。在不同处理下的MS培养基中研究了GL-2菌株下Pb和Cd对小麦生长的影响。利用盆栽试验研究了GL-2对小麦生长的促进作用。结果表明,分子生物学和生理生化鉴定GL-2菌株为路氏肠杆菌（Enterobacter ludwigii）;GL-2对重金属具有很强的耐受性,其中对Pb的最低抑制浓度高达650 mg/L;添加GL-2菌株的MS培养基可有效减轻重金属对小麦生长的影响;GL-2菌株显著增加了小麦根鲜重、分蘖数和根干重。

关键词: 路氏肠杆菌（Enterobacter ludwigii）, 小麦, 重金属, 促生作用

Abstract: In order to isolate the plant growth-promoting rhizobacteria（PGPR）that exhibit heavy metal resistance, the LB medium supplemented with Cd and the siderophores medium were used to screen suitable strains. Minimal inhibitory concentrations （MICs） of GL-2 strain was determined by plate dilution method. The passivation effects to Pb and Cd on the growth o f wheat under GL-2 strain were added to MS medium in the different treatments. The promoting effect of GL-2 was studied on the growth of wheat by pot experiments. The results showed that the strain GL-2 belongs to Enterobacter ludwigii based on molecular biology and physiological and biochemical identification. GL-2 had a strong tolerance to heavy metals, and the minimum inhibitory concentration of Pb was 650 mg/L.The MS medium added to GL-2 strains could relieve heavy metal influence on wheat growth effectively. There was a significantly effect to increase the root fresh weight, tiller numbers and root dry weight of wheat within GL-2 strains.

Key words: Enterobacter ludwigii, wheat, heavy metal, plant growth-promoting

中图分类号:

S512.1

蔡高磊, 周炀, 赵昌松, 彭宣和, 张振, 赵建宁. 耐重金属肠杆菌GL-2鉴定及对小麦促生作用的研究[J]. 湖北农业科学, 2021, 60(23): 44-48.

CAI Gao-lei, ZHOU Yang, ZHAO Chang-song, PENG Xuan-he, ZHANG zhen, ZHAO Jian-ning. Identification on heavy metal-resistant Enterobacter ludwigii GL-2 and effects on growth of wheat[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(23): 44-48.

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耐重金属肠杆菌GL-2鉴定及对小麦促生作用的研究

Identification on heavy metal-resistant Enterobacter ludwigii GL-2 and effects on growth of wheat

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