关键词: 矿质肥, 丘北辣椒, 16S rDNA, 细菌

Abstract: The effect of different dosage of mineral fertilizer on rhizosphere bacterial community of Qiubei pepper at seedling stage was compared to explore its effect on rhizosphere environment of Qiubei pepper and its application rule. 16S rDNA high-throughput sequencing was used to determine the rhizosphere soil bacterial community structure and analyze the diversity. The results showed that in the 5 treatments, the proportion of Proteobacteria decreased with the increase of the dosage of mineral fertilizer, the highest was CK （0 g/L）, which was 65.70%, and the lowest was treatment D（60 g/L）, which was 54.04%; and the Firmicutes showed an upward trend, the highest was treatment D （60 g/L）, which was 7.90%, and the lowest was treatment A （15 g/L）,which was 1.38%. In the correlation analysis with environmental factors, at the phylum level, Proteobacteria was significantly negatively correlated with N and Mg; it showed an extremely significantly negative correlation with P, Na, B, Mn and EC; Firmicutes was significantly positively correlated with Mn and EC, and had an extremely significantly positive correlation with P and B. It showed that with the increase of application, high-concentration treatment decreased the relative abundance of Proteobacteria and increased the relative abundance of Firmicutes. In addition to N and P in soil nutrients, trace elements such as Na, B and Mn also had a significant impact on the composition of pepper rhizosphere microorganisms.

Key words: mineral fertilizers, Qiubei pepper, 16S rDNA, bacteria