湖北农业科学 ›› 2024, Vol. 63 ›› Issue (7): 78-82.doi: 10.14088/j.cnki.issn0439-8114.2024.07.012

• 园艺·特产 • 上一篇    下一篇

喷施茉莉酸甲酯对油茶幼苗叶片生理生化的影响

蒋瑶, 陈文波, 雷志民   

  1. 凯里学院大健康学院,贵州 凯里 556011
  • 收稿日期:2023-04-19 出版日期:2024-07-25 发布日期:2024-07-24
  • 作者简介:蒋 瑶(1982-),女,四川德阳人,教授,博士,主要从事植物生物技术研究,(电话)18385756616(电子信箱)jiangyao0221@163.com。
  • 基金资助:
    贵州省教育厅自然科学重点项目(黔教合KY字[2015]412号); 贵州省科学技术基金项目(黔科合基础[2018]1413号); 贵州省教育高质量发展项目(农业硕士点建设)

Effects of methyl jasmonate on the physiology and biochemistry of seedlings leaves from Camellia Oleifera

JIANG Yao, CHEN Wen-bo, LEI Zhi-min   

  1. School of Life and Health Science, Kaili University, Kaili 556011, Guizhou,China
  • Received:2023-04-19 Published:2024-07-25 Online:2024-07-24

摘要: 探讨喷施茉莉酸甲酯对油茶(Camellia oleifera Abel.)幼苗叶片生理生化的影响,以湘林27号、湘林36号、长林3号和长林4号4种贵州省天柱县主栽油茶品种的2年生嫁接苗为研究材料,采用露地栽培的方法,以6种不同浓度(0、100、300、600、1 000、1 500 μmol/L)的茉莉酸甲酯处理进行试验,测定4种油茶品种叶片中抗氧化酶活性、可溶性蛋白质、可溶性糖等7个生理指标的变化情况。结果表明,100 μmol/L茉莉酸甲酯喷施处理有利于提高油茶幼苗叶片中的超氧化物歧化酶、过氧化物酶和过氧化氢酶的活性,有效降低丙二醛含量,增加可溶性蛋白质、可溶性糖和游离脯氨酸的含量。

关键词: 茉莉酸甲酯, 油茶(Camellia oleifera Abel.)幼苗叶片, 喷施, 生理生化

Abstract: To investigate the effects of spraying methyl jasmonate on the physiology and biochemistry of oil tea(Camellia oleifera) seedling leaves, 2-year-old grafted seedlings of four main oil tea varieties from Tianzhu County, Guizhou Province, including Xianglin No. 27, Xianglin No. 36, Changlin No. 3 and Changlin No. 4, were used as study materials. Six different concentrations (0, 100, 300, 600, 1 000, 1 500 μmol/L) of methyl jasmonate were used to determine the activity of antioxidant enzymes, soluble protein, soluble sugar and other 7 physiological indexes in the leaves of 4 oil tea varieties. The results showed that 100 μmol/L methyl jasmonate spraying treatment was conducive to increase the activities of superoxide dismutase, peroxidase and catalase, effectively reducing malondialdehyde content, and increase the content of soluble proteins, soluble sugars and free proline in the leaves of oil tea seedlings.

Key words: methyl jasmonate, seedlings leaves of Camellia oleifera, spaying, physiological index

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