关键词: 马铃薯（Solanum tuberosum）, Rieske FeS基因, 克隆, 生信分析, 干旱胁迫, 基因表达

Abstract: The potato (Solanum tuberosum) was used as the research object. The cDNA sequence of the Rieske FeS gene of the potato was cloned by RT-PCR technology, and bioinformatics analysis was performed on the gene. The expression of the Rieske FeS gene under simulated drought stress (0, 12, 24 h) was detected by fluorescence quantitative PCR. The results showed that the full-length cDNA sequence of the potato Rieske FeS gene was 693 bp, containing one open reading frame and encoding 230 amino acids. The molecular weight of the potato Rieske FeS protein was 24.27 ku, with a theoretical isoelectric point of 8.20;the Rieske FeS protein contained the CytB6-F_Fe-S domain (59~97 aa), transmembrane region (72~94 aa), and Rieske domain (113~203 aa);the secondary structure of potato Rieske FeS protein had the highest proportion of irregular curls, followed by β-sheets, and the lowest proportion was α - helices. The phylogenetic tree analysis showed that the potato Rieske FeS protein was most closely related to the tobacco Rieske FeS protein. The qPCR results showed that the Rieske FeS gene of the potato was expressed in all tested tissues, with the highest relative expression level of Rieske FeS gene in leaves, followed by stems and roots, and the lowest relative expression level in tubers. After PEG drought stress, the relative expression level of Rieske FeS gene in leaves showed a decreasing trend followed by an increasing trend, and the relative expression level at 12 hours was significantly lower than that at 0 hours (control group). The relative expression level of Rieske FeS gene in stem segments was significantly upregulated after 12 hours of simulated drought treatment; the relative expression level of Rieske FeS gene was significantly upregulated in tubers under simulated drought stress for 24 hours; there was no significant difference in the relative expression levels of Rieske FeS gene among the drought treatments in the roots. The potato Rieske FeS gene was closely related to drought stress.

Key words: potato（Solanum tuberosum）, Rieske FeS gene, clone, bioinformatics analysis, drought stress, gene expression