湖北农业科学 ›› 2025, Vol. 64 ›› Issue (12): 38-41.doi: 10.14088/j.cnki.issn0439-8114.2025.12.006

• 育种·栽培 • 上一篇    下一篇

云荞1号与米苦荞人工去雄杂交及其杂交后代农艺性状分析

尹桂芳, 王艳青, 孙道旺, 隆文杰, 卢文洁, 王莉花   

  1. 云南省农业科学院生物技术与种质资源研究所/云南省农业生物技术重点实验室/农业农村部西南作物基因资源与种质创制重点实验室,昆明 650205
  • 收稿日期:2024-07-02 发布日期:2025-12-30
  • 通讯作者: 王莉花(1970-),女,山西运城人,研究员,硕士,主要从事荞麦病虫害防控技术与育种研究,(电子信箱)908242246@qq.com。
  • 作者简介:尹桂芳(1981-),女,云南宣威人,副研究员,硕士,主要从事荞麦生物技术育种研究,(电子信箱)1434651675@qq.com;共同第一作者,王艳青(1977-),女,云南石屏人,高级实验师,学士,主要从事荞麦资源鉴定,(电子信箱)364669625@qq.com。
  • 基金资助:
    财政部和农业农村部:国家现代农业产业技术体系资助项目(CARS-07-C-2); 云南省重大科技专项与重点研发计划项目(202202AE090020); 云南省农业基础研究联合专项(202301BD070001-039); 云南省农业基础研究联合专项(202101BD070001-122)

Artificial emasculation and hybridization between Yunqiao No.1 and Mikuqiao, and agronomic trait analysis of their hybrid offspring

YIN Gui-fang, WANG Yan-qing, SUN Dao-wang, LONG Wen-jie, LU Wen-jie, WANG Li-hua   

  1. Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences/ Yunnan Provincial Key Laboratory of Agricultural Biotechnology/Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Kunming 650205, China
  • Received:2024-07-02 Online:2025-12-30

摘要: 为提高苦荞[Fagopyrum tataricum (L.)Gaertn.]杂交成功率及选育大子粒、易脱壳、高产苦荞品种提供参考依据,应用人工去雄杂交方法,以云荞1号(厚壳)为母本、米苦荞(薄壳)为父本进行杂交获得后代,并比较分析了父母本及杂交F2代株高、主茎节数、一级分枝数、单株粒重、单株粒数、千粒重和壳型等农艺性状。结果表明,杂交过程中选择柱头发育成熟且花药未释放的母本花朵尤为关键。F2代厚壳型群体的性状平均值与云荞1号无显著差异;与米苦荞相比,F2代薄壳型群体的株高和一级分枝数平均值显著降低,千粒重平均值显著提高;F2代单株粒重和单株粒数变异系数较大,单株粒数最大值厚壳型为2 577粒、薄壳型为3 817粒,远超母本的986粒和父本的2 035粒,其单株粒重也最大,厚壳型为49.60 g、薄壳型为45.65 g,也远超母本的18.69 g和父本的27.13 g。通过杂交,厚壳型和薄壳型F2群体性状都得到了改善,F2代厚壳型植株单株粒数和单株粒重的最大值均显著提高,薄壳型植株的千粒重显著提高,株高和一级分枝数显著降低。

关键词: 苦荞[Fagopyrum tataricum (L.) Gaertn.], 云荞1号, 米苦荞, 人工去雄, 杂交, F2代, 农艺性状

Abstract: To provide references for improving the hybridization success rate of tartary buckwheat [Fagopyrum tataricum (L.)Gaertn.]and breeding large-grain, easy-to-thresh, high-yield tartary buckwheat varieties, the artificial emasculation hybridization method was applied. Using Yunqiao No.1 (thick-shelled) as the female parent and Mikuqiao (thin-shelled) as the male parent, hybrid offspring were obtained. Agronomic traits of the parents and the F2 generation, including plant height, number of main stem nodes, number of primary branches, grain weight per plant, grain number per plant, thousand-grain weight, and shell type, were compared and analyzed. The results indicated that selecting female parent flowers with mature stigmas and anthers that have not yet dehisced was particularly crucial during the hybridization process. There were no significant differences in the mean values of traits between the thick-shelled F2 population and Yunqiao No.1. Compared to Mikuqiao, the thin-shelled F2 population showed significantly lower mean values for plant height and number of primary branches, while the mean thousand-grain weight was significantly higher. The coefficient of variations for grain weight per plant, and grain number per plant was the large among the traits in the F2 generation. The maximum grain number per plant reached 2 577 grains for the thick-shelled type and 3 817 grains for the thin-shelled type, far exceeding the 986 grains of the female parent and the 2 035 grains of the male parent. Similarly, the grain weight per plant was also the highest, with the thick-shelled type at 49.60 g and the thin-shelled type at 45.65 g, significantly exceeding the female parent’s 18.69 g and the male parent’s 27.13 g. Through hybridization, the plant traits of both thick-shelled and thin-shelled types were improved. The maximum values for grain number per plant, and grain weight per plant were significantly increased in the thick-shelled F2 plants, while the thin-shelled F2 plants showed a significant increase in thousand-grain weight and a significant decrease in plant height and number of primary branches.

Key words: tartary buckwheat[Fagopyrum tataricum (L.) Gaertn.], Yunqiao No.1, Mikuqiao, artificial emasculation, hybrid, F2 generation, agronomic trait

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