QuEChERS-超高效液相色谱-串联质谱法测定花生中氯虫苯甲酰胺和噻虫胺的残留及膳食风险评估

doi:10.14088/j.cnki.issn0439-8114.2026.09.024

湖北农业科学 ›› 2026, Vol. 65 ›› Issue (9): 147-151.doi: 10.14088/j.cnki.issn0439-8114.2026.09.024

QuEChERS-超高效液相色谱-串联质谱法测定花生中氯虫苯甲酰胺和噻虫胺的残留及膳食风险评估

朱坤淼¹, 张淑娜², 陈鑫¹, 程璐瑶¹, 王靖捷¹, 吕昂¹, 刘军¹

1.湖北省农业科学院农业质量标准与检测技术研究所/农产品营养品质与安全湖北省重点实验室,武汉 430064;
2.武汉问津职业学校,武汉 430010

收稿日期:2026-05-11 出版日期:2026-09-25 发布日期:2026-09-17
通讯作者: 吕昂(1987-),男,湖北孝感人,助理研究员,博士,主要从事农药登记残留试验及农产品质量安全研究,（电子信箱）lvang@hbaas.ac.cn;共同通信作者,刘军(1979-),男,安徽寿县人,副研究员,硕士,主要从事农产品质量安全研究,（电子信箱）liujun@hbaas.ac.cn。
作者简介:朱坤淼（1992-）,男,河南叶县人,助理研究员,博士,主要从事农药登记残留试验及农产品质量安全研究,（电子信箱）zhukunmiao@hbaas.ac.cn;共同第一作者,张淑娜(1993-),女,河南沈丘人,硕士,主要从事农产品质量安全研究,（电子信箱）1160987038@qq.com
基金资助:
湖北省农业科学院青年基金项目(2024NKYJJ32);湖北省技术创新计划项目（2024BBB098）

Determination of chlorantraniliprole and clothianidin residues in peanuts by QuEChERS-ultra-high performance liquid chromatography-tandem mass spectrometry and dietary risk assessment

ZHU Kun-miao¹, ZHANG Shu-na², CHEN Xin¹, CHENG Lu-yao¹, WANG Jing-jie¹, LYU Ang¹, LIU Jun¹

1. Institute of Agricultural Quality Standards and Testing Technology Research, Hubei Academy of Agricultural Sciences / Hubei Key Laboratory of Nutritional Quality and Safety of Agro-Products, Wuhan 430064, China;
2. Wuhan Wenjin Vocational School, Wuhan 430010, China

Received:2026-05-11 Published:2026-09-25 Online:2026-09-17

摘要/Abstract

摘要： 为了建立花生仁和秸秆中氯虫苯甲酰胺和噻虫胺残留的同时检测方法并评估其膳食风险,基于QuEChERS前处理技术,结合超高效液相色谱-串联质谱（UPLC-MS/MS）,通过优化提取盐包与净化管组合,在电喷雾正离子模式及多反应监测模式下进行检测,外标法定量,并对中国10个地区花生样品进行残留测定及慢性膳食风险评估。结果表明,氯虫苯甲酰胺和噻虫胺在0.002 5~0.200 0 mg/L范围内线性关系良好,相关系数（R²）均大于0.990 0。花生仁中氯虫苯甲酰胺的平均回收率为81.8%~88.0%,相对标准偏差（RSD）为2.2%~6.1%;噻虫胺的平均回收率为77.0%~82.4%,RSD为5.1%~12.6%。秸秆中氯虫苯甲酰胺平均回收率为73.0%~79.6%,RSD为1.4%~4.9%;噻虫胺平均回收率为78.0%~92.8%,RSD为0.9%~7.9%。10个地区花生仁中两种农药的残留量均未超出《食品安全国家标准食品中农药最大残留限量》限量标准。慢性膳食风险评估显示,氯虫苯甲酰胺和噻虫胺的风险商分别为4.6%和6.6%,均远低于100%。该方法简便高效,灵敏度高,且具有良好的准确度与精密度,适用于花生中两种农药残留的同步检测。

关键词: QuEChERS, 超高效液相色谱-串联质谱法（UPLC-MS/MS）, 花生, 氯虫苯甲酰胺, 噻虫胺, 残留, 膳食风险

Abstract: To establish a simultaneous detection method for chlorantraniliprole and clothianidin residues in peanut kernels and straw and to assess their dietary risk, this study used QuEChERS pretreatment technology combined with ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), optimized the combination of extraction salt packets and purification tubes, performed detection in electrospray positive ion mode and multiple reaction monitoring mode, quantified by the external standard method, and conducted residue determination and chronic dietary risk assessment for peanut samples from 10 regions of China.The results showed that chlorantraniliprole and clothianidin had good linear relationships in the range of 0.002 5 to 0.200 0 mg/L, with correlation coefficients (R²) all greater than 0.990 0. The average recoveries of chlorantraniliprole in peanut kernels ranged from 81.8% to 88.0%, with relative standard deviations (RSD) of 2.2% to 6.1%; the average recoveries of clothianidin ranged from 77.0% to 82.4%, with RSD of 5.1% to 12.6%. The average recoveries of chlorantraniliprole in straw ranged from 73.0% to 79.6%, with RSD of 1.4% to 4.9%; the average recoveries of clothianidin ranged from 78.0% to 92.8%, with RSD of 0.9% to 7.9%. The residues of both pesticides in peanut kernels from 10 regions did not exceed the limits specified in the National food safety standard-Maximum residue limits for pesticides in food. Chronic dietary risk assessment showed that the risk quotients of chlorantraniliprole and clothianidin were 4.6% and 6.6%, respectively, both far below 100%. The method was simple and efficient, had high sensitivity, exhibited good accuracy and precision, and was suitable for the simultaneous detection of the two pesticide residues in peanuts.

Key words: QuEChERS, ultra-high performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS）, peanut, chlorantraniliprole, clothianidin, residue, dietary risk

中图分类号:

TQ450.2

朱坤淼, 张淑娜, 陈鑫, 程璐瑶, 王靖捷, 吕昂, 刘军. QuEChERS-超高效液相色谱-串联质谱法测定花生中氯虫苯甲酰胺和噻虫胺的残留及膳食风险评估[J]. 湖北农业科学, 2026, 65(9): 147-151.

ZHU Kun-miao, ZHANG Shu-na, CHEN Xin, CHENG Lu-yao, WANG Jing-jie, LYU Ang, LIU Jun. Determination of chlorantraniliprole and clothianidin residues in peanuts by QuEChERS-ultra-high performance liquid chromatography-tandem mass spectrometry and dietary risk assessment[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(9): 147-151.

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QuEChERS-超高效液相色谱-串联质谱法测定花生中氯虫苯甲酰胺和噻虫胺的残留及膳食风险评估

Determination of chlorantraniliprole and clothianidin residues in peanuts by QuEChERS-ultra-high performance liquid chromatography-tandem mass spectrometry and dietary risk assessment

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