关键词: 水产品, 硝基咪唑类药物, 高效液相色谱-串联质谱法, 药物残留量

Abstract: A high-performance liquid chromatography-tandem mass spectrometry method was established for the detection of nitroimidazole drugs residues in aquatic products. The sample was extracted with ethyl acetate, distilled under reduced pressure, purified with n-hexane, and the residue was dissolved in 10% methanol before passing through a C18 chromatographic column （150 mm×2 mm, 5 μm）. Separation was carried out using 0.20% formic acid aqueous solution acetonitrile as the mobile phase, gradient elution was performed, and the target compound was analyzed using high-performance liquid chromatography tandem mass spectrometry. The detection limit of the method was 0.04~0.30 μg/kg, the quantitative limit was 0.50~1.00 μg/kg, the linear relationship was good within the range of 1~150 μg/L, and the correlation coefficient （R²） was >0.998 00. At spiked level of 5.0, 7.5, 10.0, 15.0, and 20.0 μg/kg, the recovery rate was 73.0%~116.2%, and the relative standard deviation （RSD） was 0.43%~9.53%. This method was easy to operate, highly sensitive, and had good reproducibility, making it suitable for the detection of large batches of samples.

Key words: aquatic products, nitroimidazole drugs, high performance liquid chromatography tandem mass spectrometry, drugs residues