Response of Arabidopsis AT4G32010 Gene Deleted Mutant to Abiotic Stress

doi:10.14088/j.cnki.issn0439-8114.2018.13.025

Abstract

Abstract: B3 family genes are unique transcription factors in plants and participate in the process of plant stress resistance. Arabidopsis thaliana HSI gene（AT4G32010） is a member of B3 family. In this study, homozygous mutant hsi were obtained by using PCR. The expression of HSI gene in mutant is lower than that in wild type. Under drought and salt stress, the germination rate of wild type and mutant hsi decreased with the increase of mannitol and salt concentration. At the same concentration, the germination rate of the mutant hsi was lower than that of the wild type, and the difference was significant. The results indicated that HSI gene of Arabidopsis thaliana played a positive role in seed germination. Under drought and salt stress, there was no significant difference in root length between wild type and mutant. It is indicated that the HSI gene is involved in the growth process of the root, and the regulatory role of the gene needs to be further clarified.

Key words: Arabidopsis thaliana, B3 family gene, HSL1 gene, drought stress, salt stress

CLC Number:

Q789

ZHAO Yan-min, WENG Qiao-yun. Response of Arabidopsis AT4G32010 Gene Deleted Mutant to Abiotic Stress[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(13): 94-97.

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