Selecting of Antagonistic Bacillus against Tobacco Black Shank and Detecting of Control Efficacy

doi:10.14088/j.cnki.issn0439-8114.2018.18.014

Abstract

Abstract: In order to control the tobacco black shank caused by Phytophthora parasitica var. nicotianae,42 bacterial strains were isolated from different health status tobacco rhizosphere soil,10 of them had an antagonistic effect on P. nicotianae by using the plate confronting method and treatment with fermentation filtrate after heated at 115 ℃. Three groups of Bacillus could be identified under the 16S rDNA sequence analysis：Bacillus amyloliquefaciens, B. subtilis and B. velezensis. The characteristics of cellulase and protease activity,colonization of Bacillus in tobacco rhizosphere soil,root and stem,and field experiments were carried out on B. subtilis GJ1,B. amyloliquefaciens GJ7 and B. velezensis GJ11. The results showed that GJ1,GJ7 and GJ11 strains could secrete cellulase and protease,and colonize in the rhizosphere soil,root and stem of tobacco. The ability of colonization in the rhizosphere soil was GJ1>GJ7>GJ11,in root and stem was GJ7>GJ1>GJ11. However,the control efficiency against tobacco black shank of GJ1,GJ7 and GJ11 were 33.33%,80.00% and 28.60%,respectively,suggesting that the control efficacy in field of antagonistic Bacillus may depend on its colonization ability in tobacco.

Key words: Phytophthora parasitica, antagonistic bacteria, colonization, control efficacy

CLC Number:

S476
S435.72

SHI He-li, TAN Jun, DING Cai-fu, ZHAO Xiu-yun, XIANG Bi-kun, LUO Fang. Selecting of Antagonistic Bacillus against Tobacco Black Shank and Detecting of Control Efficacy[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(18): 60-65.

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