HUBEI AGRICULTURAL SCIENCES ›› 2019, Vol. 58 ›› Issue (2): 107-110.doi: 10.14088/j.cnki.issn0439-8114.2019.02.024

• Detection Analysis • Previous Articles     Next Articles

Development of A Rapid Test Strip for Determination of Metalaxyl Residues in Tobacco

YUAN Guang-yu, GONG Wei-yao, LUO Wei-chao, XU Ming-hui, JIA Ling-ling, XIE Ti-bo, ZHONG Xin-min, CHENG Ru   

  1. Guizhou Kwinbon Science and Technology for Food Safety Co.,Ltd.,Guiyang 550009,China
  • Received:2018-06-29 Online:2019-01-25 Published:2019-11-26

Abstract: : In order to develop a method for rapid detection of metalaxyl residues in tobacco, a colloidal gold immunochromatography test strip was developea by using the self-made antigen and antibody as the material. The hapten was synthesized from metalaxyl and further prepared monoclonal antibody against metalaxyl after antigen immunization. The test strip limits the test limit to dry tobacco 1.5 mg/kg, fresh tobacco leaves 1.0 mg/kg, test paper stable, no cross reaction with other drugs, and the comparison with the chromatographic method is 98%, false positive rate is 2.3%. It can be used to detect tobacco quickly, accurately and batch, and provide technical support for the detection of methfrol residue in tobacco.

Key words: tobacco(Nicotiana tabacum L.), metalaxyl, test strip, fast detection

CLC Number: